Osteogenic Differentiation Of Cord Blood Stromal Cells On Composite Polymer Scaffolds | 4666
Journal of Biotechnology & Biomaterials
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Composite polymers (PLGA) scaffolds were fabricated by solvent casting and simultaneous particulate leaching, and their ability to enhance the osteoblastic behavior of umbilical cord blood stromal cells (MSCs) in osteogenic media was investigated. MSCs were isolated from human umbilical cord blood and cultured on polymer scaffolds to assess short-term
cyto- compatibility and long-term physiological behavior. Smooth polypropylene flasks were used as control surfaces. The short-term cyto-compatibility results indicated that hybrid polymer scaffolds supported comparatively good cell adhesion and viability compared with control surfaces. In osteogenic conditions, MSCs grown on this polymer scaffolds also shown increased levels of alkaline phosphatase activity after 3 weeks of culture. Calcium phosphate mineralization was substantially accelerated over the scaffolds compared to control surfaces as indicated through von Kossa and calcium staining. These results demonstrate the enhanced tissue regeneration property of hybrid composite scaffolds, which may be of potential use for engineering osteogenic
scaffolds for orthopedic applications.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals