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Pectinases Purification And Biotechnological Applications Of Fermented Wastes From Thermomyces Lanuginosus | 4795
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
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Pectinases purification and biotechnological applications of fermented wastes from Thermomyces lanuginosus

3rd World Congress on Biotechnology

Essam A. Makky and Mashitah M. Yusoff

ScientificTracks Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.009

A pectin degradation enzymes are widely used in the industrial processing of fruits and vegetables in order to decrease the viscosity of juices and thus to facilitate extraction, maceration, liquefaction, filtration and clarification processes. Sugar-cane bagasse (SCB) was used as the substrate for pectinases from T. lanuginosus at 55?C under solid-state fermentation (SSF) culture. Exo-PG and total PNL & PL enzymes were purified using Gel-filtration chromatography; the former exhibited only one sharp peak, while the latter showed two identical peaks each of them represents one enzyme. Biotechnological applications of fermented bagasse obtained mixed with the fungal biomass at 55?C as biofertilizer at different concentrations (w/w) were cultivated with Zea mays for 30 days to indicate the growth on sandy soil and succeeded to induce the plant growth which may give an indication of applying the present biofertilizer particularly in the reclaimed sandy soils. Only 14 and 13 amino acids were detected and obviously that Exo-PG was typical proline containing enzyme and total PNL & PL were glycine/histidine containing enzymes, respectively.