Preparation Of Emulsified Encapsulated Egg Albumin Nanoparticles Of Azadirachta Indica Alkaline Phosphatase | 4811
Journal of Biotechnology & Biomaterials
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Nanoparticles are spherical particles which allow the controlled release of active ingredient. Nanoparticles have been used as
carriers in the delivery of drugs, antigens, hormones, enzymes and genes. Besides, it has many advantages in the industrial
application. Albumin is a major plasma protein constituent and its biodegradation leads to form natural products such as amino
acids (tyrosine, tryptophan & asparagines). As well as these, albumin nanoparticles are non-toxic and non-antigenic in nature.
Albumin nanoparticles are easily metabolized in the body as well as the size of particles, degree of stabilization. Hence, its colloidal
forms have been considered as potential carriers of drugs for their site-specific localization. Thus, albumin, being biodegradable,
biocompatible, and nontoxic, was chosen as a matrix material for the preparation of the nanoparticles. Our work involves usage
of egg albumin for entrapment of biologically active materials Azadirachta indica alkaline phosphatase using cross linking agent
(glutaraldehyde) and emulsifier (coconut oil).Glutaraldehyde is used as cross linking agent and coconut oil is used as emulsifier
which given the newly formed fluorescent complex. The oil bath was prepared by adding glutaraldehyde and n-butanol in
coconut oil along with 50 U of Azadirachta indica alkaline phosphatase. This solution was filled in the syringe and dispersed
in prepared oil bath as well as stirred for overnight at 37?C. Centrifuged at 5000rpm for 10min and supernatant was removed
and pellet was collected which contain prepared nanoparticles. These prepared nanoparticles were washed with cold ether and
acetone. The emulsification with coconut oil was increased the stability of encapsulated nanoparticles as well as responsible for
controlled release of enzyme in the delivery system in the presence of proteases. The prepared nanoparticles were spherical in
shape and off-white to whitish brown in colour. The specific activity of Azadirachta indica alkaline phosphatase was studied at
different time intervals (1 to 7 days)as well as using different concentration of proteases such as trypsin, chymotrypsin and papin
( 6U, 12 U & 18U) to understand the biodegradibilty of the cross linked entrapped Azadirachta indica alkaline phosphatase. The
enzyme activity of Azadirachta indica alkaline phosphatase in emulsified encapsulated egg albumin nanoparticles was estimated
by p-nitrophenyl-phosphate method. Among them the chymotrypsin was found to be the best proteolytic enzymes which release
the maximum activity of Azadirachta indica alkaline phosphatase in solution at the fifth day, thereby confirming the proper
immobilization of enzyme in prepared nanoparticles of egg albumin under specified conditions. In the absence of proteolytic
enzymes, Azadirachta indica alkaline phosphatase was not detected. This immobilized Azadirachta indica alkaline phosphatase
in emulsified encapsulated egg albumin nanoparticles can be used in detergent, leather, food and pharmaceutical industries for
washing of fabrics as well as preservative in different kind of syrups preparation.
Kirti Rani has done her M.Sc. in 2000 and completed her Ph.D (2004) at the age of 25 years from Department of Biochemistry, Maharshi Dayanand
University, Rohtak, India. She is ex-senior resident/demonstrator of department of Biochemistry, Post-graduate Institute of Medical Education &
Research, PGIMER, Chandigarh, India (2004-2007). She has also worked as lecturer 7 invited as guest faculty in others private/Govt. affiliated
Indian colleges/institutions (2007-2008). She is Assistant Professor in Amity Institute of Biotechnology, Amity University, Noida, India (2008-till date).
She has more than 25 international & national publications including research articles/ monographs/ books/ patents/ abstracts. She is also member
of Society of Biological Chemists, India and Stem Cell Voice of India. She is also reviewer of more than 8 international journals.
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