Production And Purification Of Phytase By Solid State Fermentation From Rhizopus Oligosporus MTCC 556 Using Agricultural Wastes | 4714
Journal of Biotechnology & Biomaterials
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Phytic acid (myo-inositol 1,6 hexakisphosphate) is the major storage form of phosphorus in plants. It cannot be metabolized
in monogastric animals since phytic acid acts as an anti-nutritional agent by chelating several metal ions and insoluble
form of protein complex. This consequence resulted in mineral deficiencies and phosphorus pollution. One of the finest ways
to utilize the phosphorus from phytate is the use of phytase enzyme. In this study, Rhizopus oligosporus MTCC 556 was used
to produce phytase by solid state fermentation (SSF) using six different agricultural residues as substrates. A maximal enzyme
production of 15.8 U/gds was observed with wheat bran as substrate. Three-step purification was employed with ammonium
sulfate precipitation, ion exchange and gel filtration chromatography which resulted in 14.6-fold purified phytase. The molecular
weight was estimated to be 88Kda by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimum
pH and temperature for phytase activity was found to be 5.5 and 55?C respectively. The effect of different metal ions was examined
which showed that Mg
ions enhanced the phytase production significantly. This study would be useful for reducing
the phosphorus pollution and increasing interest towards efficient utilization of agro industrial wastes for enzyme production.
Suresh S is pursuing his PhD in the department of chemical engineering, Anna University, Chennai. He has completed M.Sc (Biochemistry) during
2007-09 at SRM Arts and Science College, Chennai and also having a teaching experience for one year at Dr. MGR.Chockalingam Arts College,
Arni, Thiruvanamalai District, Tamilnadu.
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