Production Of Cellulase By Cellulomonas Uda Using Banana Fruit Stalk Under Solid Substrate Fermentation | 8515
Journal of Biotechnology & Biomaterials
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Research on cellulase has progressed very rapidly in
the past few decades?, emphasis being in enzymatic
hydrolysis of cellulose to glucose. Development of an
economical process for cellulase production is hindered
because of high cost of the substrate (pure cellulose) and
some of the chemicals such as protease, peptone and
because of low yields of cellulase per unit of cellulose.
To overcome these bottlenecks, cheap source are used
which require minimum pre-treatment and purification
and increased cellulase yields per unit of cellulose.
Banana (Musa sopiestum) fruit stalk abundantly
available in banana production fields and markets,
appear to be favorable substrate as it is cheaply available
in the tropical and sub-tropical countries and has
a cellulose content of 23.85%. It accumulates in the
agro industrial yards, has no significant industrial and
commercial uses but contribute serious environmental
problems. According to 2009 NABARD statistics average
yield of banana per hectare per year is 30.63 tonnes
in India. Thus contributing to abundant availability of
Banana fruit stalks which can be used as substrate in
solid substrate fermentation.
Cellulomonas uda is an actinomycetes which is capable
of feeding on cellulose as carbon source. To date, the
production of cellulase has been widely studied in
submerged culture process but the relatively high cost
of enzyme production has hindered the industrial
application of cellulose bioconversion. Adopting
Solid substrate fermentation is an attractive process to
produce cellulose economically due to its lower capital
investment and lower expenses.
In our present work, Production of cellulase by
Cellulomonas uda by using banana fruit stalk under
solid state fermentation, the activity of cellulase
obtained is 6.39 U/ml at an optimum conditions of pH
7.79, temperature 350C, incubation time 96 hrs .
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