Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
GET THE APP
Promoters Promoting Transgenic Research | 4856
ISSN: 2155-952X
Journal of Biotechnology & Biomaterials
Open Access
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Plant can be used as an alternative expression system for production of recombinant proteins due to reduced production
cost and lower capital investment along with appropriate posttranslational modification. One of the major limitations in
moving plant-produced heterologous proteins from developmental research to commercial products is lower level of expression
of transgene in plant. Expression levels of transgene/gene of interest depend upon strength of genetic regulator, the promoter. In
plant molecular pharming, strong promoters may be needed to achieve maximal expression of multiple genes by successive event
of transformations. However, a risk of multiple transformations by a single promoter is that, it might lead to silencing of both
genes in successive generation through homologous recombination. Considering all the facts, attempts are being made worldwide
to develop engineered promoter with enhanced strength and heterogeneous sequences employing different approaches including
promoter DNA hybridization and shuffling. Recently, adopting these novel techniques we have developed several useful promoters
viz., MSgt-FSgt, FuasFScp, MUAS35SCP. They showed 5-8 times stronger activity in transgenic tobacco and Arabidopsis as
compared to CaMV35S, a widely used promoter in plant molecular biology. Functional activities of such promoters were further
characterized in animal cell line using different cytokines such as IL-33 and IL-35. Our observation clearly indicated that these
promoters are active not only in plant but also in animal cell line at greater level. The genetic tools developed by our group could
be potential for controlling expression of various genes in both transgenic plants and animal.
Biography
Relevant Topics
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals