Journal of Biotechnology & Biomaterials
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The damage caused by free radicals and their resulting oxidative stress on the living cells has been studied extensively
in the recent years. Free radicals have been demonstrated to be the main initiator for many disease s including cancer,
neurodegenerative disease etc. As reported earlier, Protein Protease Inhibitors (PPIs) are said to have anti-oxidant capacity
and are believed to play roles in various biological activities. Therefore, the present study aims to continue the investigation of
anti-oxidant property of PPI in Cajanuscajan andPhaseoluslimentuswhich being a legume serve as a rich source for protein
and hence, yield of PPI is higher as compared to other sources. C. cajan(PUSA-992) variety was homogenized, defatted and
precipitated with ammonium sulphate. The precipitate (30-50%) was dialyzed and chromatographed on DEAE-cellulose
column to obtain Cajanuscajan Trypsin Inhibitor (CCTI). PPI from Phaseoluslimentus (Lima Bean Trypsin Inhibitor/ LBTI)
was purchased commercially from Sigma Aldrich. The anti-oxidant activity of both the PPIs was analyzed by two most
common radical scavenging assays of FRAP (ferric reducing antioxidant power) and DPPH (1,1-diphenyl-2-picrylhydrazyl).
The results indicated that the PPIs were potent with direct anti-oxidant activity in both DPPH and FRAP assays. The CCTI and
LBTIshowed DPPH radical scavenging activity in a concentration-dependent manner with IC50 values 544 μg/ml and 506 μg/
ml. Also, FRAP assay showed ferric ion reducing ability of LBTI and CCTI with highest values of 0.226mM/mg and 0.218mM/
mg FRAP value respectively as compared to FRAP value of standard i.e. 0.258mM/mg.
Sadaf Fatima completed her PhD from Interdisciplinary Biotechnology Unit, Aligarh Muslim University, India, on Protein Folding and Stability. She is now working
as Assistant Professor in Jamia Millia Islamia, New Delhi, India. She has published 11 research articles. 2 review articles and 2 articles are in press. Three PhD
students are working under her guidance. She is running a project from DST on ?Purification, characterization and crystallization of Protein Protease Inhibitors
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