Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar


Proteomic Analysis Of Milk Is Indicative Of Host Immune Response To Mastitis In Buffaloes | 4885
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 2154

Journal of Biotechnology & Biomaterials received 2154 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Recommended Journals
Share This Page

Proteomic analysis of milk is indicative of host immune response to mastitis in buffaloes

3rd World Congress on Biotechnology

S K Phulia1, AK Balhara, I Singh, Varij Nayan, RK Sharma, Sunesh and AK Mohanty

ScientificTracks Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.009

The unique ability of modern proteomics techniques offers a great opportunity for studying simultaneously all proteins in a sample. The present study was undertaken to examine proteome changes in milk in response to the severity of mastitis vis-?-vis normal milk in Murrah buffaloes. Milk was collected by hand milking and mastitis was detected by California Mastitis Test (CMT), followed by milk culture to identify micro flora. Samples collected from 4 normal and 13 CMT positive samples from organised herd at the Central Institute for Research on Buffaloes, Hisar (India) were classified as normal (4), CMT positive no abnormality detected in culture (NAD; 2), subclinical (5; Streptococci infection), subclinical mixed (2; Streptococci and Staphylocci infection) and clinical (4; Streptococci) infection. Fat removed samples were subjected to reduction of pH (~ 4.0) for casein removal, followed by sample preparation for two dimensional gel electrophoresis (2 DE). Samples (equal volume of proteins from individual samples) were pooled to make single sample for all proteomic studies. Isoelectric focussing (IEF) was accomplished using broad range (pH 3-10; 7cm) dry IPG strips at 5750 Volt- hour (Vh) followed by a 12.5% SDS-PAGE. The commisse blue stained gels so obtained were densitometrically analysed by ImageMaster 2D Platinum 7.0 software (GE Healthcare, USA) to visualize % volume changes in proteins embedded in spots of different categories. Taking normal milk as reference, a total of 17 spots ? 8 from subclinical, 5 from subclinical mixed, 3 from clinical and 1 from NAD were earmarked from a total of 110 matched spots which showed a change of ≥ 5 times. These differential exhibitions in protein profile of each category suggest that the udder immune system behave differently according to stage and type of infection. Mass spectrometric analysis of the proteins in differentially expressed spots can help in identifying these proteins which can further our understanding of the host immune response to mastitis.
S K Phulia is currently working as senior scientist in the Buffalo Physiology and Reproduction division of Central Institute for Research on Buffaloes (ICAR), Hisar, Haryana. He has earlier worked in the CIRG, Makhdoom. He has completed his master?s degree in Animal physiology from National Dairy Research Institute, Karnal and Ph.D. from CCSHAU, Hisar. He has done extensive research in lactation physiology, ultrasonography of mammary gland and reproductive systems in buffaloes. He is presently working in the area of milk and mammary gland proteomics and embryo transfer technology in buffaloes.