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Proteomic Comparative Studies Among Conventional And GM Varieties Of Soybean And Maize | 17064
Journal of Biotechnology & Biomaterials
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Substantial equivalence (SE) is the first parameter of safety among conventional and genetic modified (GM) plant used
as feed and food. Although SE is not per se an effective prove of safe modification, some other methodology are on the
protocol to detect GM products, most of it are based on immunochemical reactions and PCR. Those methods are not able to
detect changes on the protein expression after the modifications. Proteomic techniques are the elected methods to identify
single or multiple changes on the protein contend in different tissues of a GM plant. In this work, assays with SDS-PAGE,
bidimensional electrophoresis (2D), and mass spectrometry used on convectional and GM seeds of soybean and maize
permitted to establish the degree of similarity on individual protein maps of each sample. It was performed the comparison
between six seed samples of commercial varieties of soybeans, and five samples of maize. Landraces of maize and bio soybean
was used as extra standards. The gel images were analyzed by ImageMaster 2D Platinum software provided by GE Healthcare.
Parameters of matching, abundance, salience, and the number of detected spots were analyzed. The results showed high level
of similarity among conventional and GM crops, but distinct if compared with bio soybeans and landraces of maize. Mass
spectrometry also confirmed the identification of the main and minor spots developed by 2D. The results support the safety of
the protein contendon GM and their isogenic counterpart seeds.
Flavio Finardi-Filho is an Associate Professor at Department of Food and Experimental Nutrition, at University of Sao Paulo, MSc and PhD in Food Science
(University of Sao Paulo); he was Visitor Researcher at FDA - Washington, DC, Research Fellow at University of California, Davis, Associate Researcher in Plant
Molecular Biology at University of California, San Diego, Research Fellow in Biotechnology at Universidad Polit?cnica, Madrid and Visitor Researcher at University
of Nottingham, UK. His research interests are: GMO and Food Safety. She is the President of the Brazilian National Technical Commission of Biosafety - CTNBio
(March, 2012-March, 2014).
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