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Quantitative Real-time PCR Compared With Pp65 Antigen Detection For Human Cytomegalovirus (HCMV) In Isfahan Province | 14130
ISSN: 2155-952X
Journal of Biotechnology & Biomaterials
Open Access
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Human cytomegalovirus: HCMV is a member of
herpes virus family and its early diagnosis could be very
critical in kidney transplant patients. It is because of the
wide range of HCMV infection clinical appearances
from tissue rejection to extremely death. Th e goal of
this study is the comparison of two common methods
of HCMV monitoring (real-time PCR and pp65 antigen
assay) to discover the accuracy of these methods.
In this study, we extract the genomic DNA of 135
kidney transplant patients from their total blood in
the Isfahan privince. HCMV monitoring was done
according to Taq Man probe scanning and pp65 surface
antigen detection using real-time PCR and pp65 antigen
assay respectively.
Th e results of our study indicated that real-time
PCR shows positive results in 36 patients (27%) but in
pp65 antigen assay 29 patients (21%) detected as HCMV
positive. It was also clear the maximum infection of
HCMV belong to spring (28%) and the minimum rate of
virus infection appear in winter (16%) but no signifi cant
association detected between HCMV infection and
seasonal patterns.
Our results indicated that real-time PCR is more
accurate than pp65 antigen assay to detect HCMV
infection in kidney transplant patients. We could also
shown that the maximum infection of HCMV belong to
spring but it was not signifi cant.
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