RAPD Analysis Of Brinjal (Solanum Melongena L) Genotypes For Polymorphism Associated With Bacterial Wilt Resistance | 8495
Journal of Biotechnology & Biomaterials
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Bacterial wilt disease caused by Ralstonia
solanacearum (Smith) Yabuuchi et al is one of
the major problems for brinjal cultivation all over the
world.Use of resistant varieties eliminates the excessive
use of poisonous chemicals in controlling the disease
which inturn reduces environmental pollution caused
by them.Surya is a bacterial wilt resistant variety of
brinjal developed by Kerala Agricultural University.
The source of resistance in this variety could be used
to develop transgenics not only in brinjal but also in
other susceptible genotypes. The present study was
undertaken to develop a molecular marker linked
to the bacterial wilt resistance gene in Surya .RAPD
analysis of the same was done with Pusa Purple
Long, a susceptible variety released from IARI. DNA
isolation was done using Rogers and Bendich (1994)
method. The purity of DNA was further analysed using
Nanodrop Spectrophotometry. Good quality DNA of
UV absorbance ratio (A260/A280) in the range of 1.8-
2 was used for study. Sixty random decamer primers
from Operon series were used for PCR amplification
to detect polymorphism between selected varieties. The
primer OPS 17 has given a polymorphic band of 1375 bp
in resistant variety.
Somya .P.P did her BSc Agriculture from College of Agriculture,Padannakkad. Now she
is pursuing her Masters degree in Agricultural Biotechnology from College of Horticulture,
Thrissur under the guidance of Dr .P.A.Valsala.
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