Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Rapid And Direct Analysis Of Small Molecular Weight Extra Polysaccharides Of Bacteria By Hi-Speed Centrifugation Coupled With Nanoparticle-assisted Laser Desorption/ionization (HC-Np-ALDI-MS) Mass Spectrometry | 4631
Journal of Biotechnology & Biomaterials
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Rapid and direct analysis of small molecular weight extra polysaccharides of Bacteria by Hi-Speed centrifugation coupled with nanoparticle-assisted laser desorption/ionization (HC-Np-ALDI-MS) mass spectrometry
The analysis of extracellular polymeric substances (EPS) from bacteria traditional methods requires chemical treatments
and complicated separation procedures. Analysis of Low molecular weight compounds on Convention MALDI-MS is very difficult due to conventional organic matrixes like DHB or CHCA matrix background peaks are interfere with samples peaks
at low molecular range .For avoiding those problems we developed a method, combining Hi-Speed centrifugation or physical extraction method with Nano-particle assisted laser desorption/ionization (HC-Np-ALDI-MS) mass spectrometry (MS) without any chemical treatment or extraction methods, separation and purification of EPS. The objective of this study is to demonstrate the use of Hi-Speed centrifugation coupled with Nanoparticle-assisted laser desorption/ionization (NALDI) mass spectrometry (MS) as a powerful technique for rapid, simple and direct analysis of low molecular weight EPS secreted by bacteria. For this we prepared manganese oxide nanoparticles and developed a nano-PALDI MS method to analyze EPS extract from different bacteria?s
by using Hi-Speed centrifugation. Our studies showed that nano-PALDI MS was selective for analysis of low molecular weight EPS. This study is a preliminary study reporting for the first time the feasibility of nano-PALDI MS techniques for the analysis of bacterial EPS without prior complicated purification and separation methodologies. The MnO2 Nano-particles working as matrix during MALDI-MS analysis and help to ionize the EPS Samples. The mass spectra did not show any background interference in the low-m/z range. The results reporting the successful use of the manganese dioxide particles as matrices for analysis of bacterial EPS has been outlined.
Gangaraju Gedda has a Masters in Analytical chemistry from JNTUH, India. He is currently a Ph.D student in Marine Biotechnology at National Sun-
Yat Sen University, Taiwan. He has published articles on the use of electrospray ionization (ESI) and on the use of matrix-assisted laser desorption/
ionization (MALDI) for mass-spectrometry-based measurement of the molecular weights of EPS molecules within bacteria. His current research
focuses on synthesizing nanomaterials, including nanowires and Nano wire based bio-chip, for use as bio-chip-based Mass Sensors. He is using
these sensors to analyze pathogenic bacteria, to perform matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals