Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Recovery And Evaluation Of Endoglucanase And Xylanase Enzymes In Agroinfiltrated Nicotiana Benthamiana Leaves | 12221
Journal of Biotechnology & Biomaterials
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
An economical method for the conversion of renewable biomass (cellulose and hemicellulose) to monosaccharide
components, which can be fermented to produce biofuels through saccharification, is to use plants as bioreactors for
cellulases production. The present work, was focused in the recovery and analysis of Endo-?-D-xylanase (EC 220.127.116.11), both
individually and in co-expression with Endo-?-1, 4-glucanase (EC 18.104.22.168) in the same Nicotiana benthamiana tissue. The genes
of interest were delivered into the leaves by vacuum agroinfiltration.
We evaluated in detached N. benthamina leaves, the expression of xylanase, using two kinds of plasmids; the first acts under
the control of the constitutive CaMV 35S promoter (pDX:xyl), and the second is a virus-based expression system (CMVva:xyl).
We also performed co-infiltration of xylanase with 3 differente gene silencing supressors (GSS): p1, p19 and p25. As a result, at
4 days post-infiltration, the xylanase expression was higher when coinfiltration with p19 and using the pDX:xyl yielding up to
17.22mg/kg FW and 0.3% of the TSP. The co-expression xylanase-endoglucanase in the same tissue was possible, with a level of
16.3mg/kg FW (0.2%TSP) and 17.18mg/kg FW (0.21% TSP) respectively, when performed in coinfiltration with p19.
The activity of the xylanase under different storage conditions (RT, 4?C, -20?C and -80?C) was estimated, after 0, 7 and 21
days. Our results indicate that the activity of the xylanase is stable for at least 21days, preserving more that 88% of the activity at
RT and -80?C storage, and 80% at 4?C and -20?C.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals