alexa Reduction Of Estrogenic Activity Of BPA By Fungal Laccase | 17148
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

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5th World Congress on Biotechnology
June 25-27, 2014 Valencia Conference Centre, Valencia, Spain

Alexandre Nunes Ponezi, Val?ria de Lima Jardim, Adilson Sartoratto and Ana L?cia Tasca Go?s Ruiz
ScientificTracks Abstracts: J Biotechnol Biomater
DOI: 10.4172/2155-952X.S1.027
Abstract
TMany chemicals have been considered as possibly for endocrine disorders described in animals and humans, such as decreased of sperm count, incidence increased of cancer and congenital malformations in the male reproductive system. Among the major Endocrine Disruptor Chemicals (EDCs), Bisphenol - A is one substance of greater production and use around the world. In vivo and in vitro tests performed with laccase from Pleurotusostreatus and Trametes sp. show that there was a reduction of more than 90% of the initial concentration (30mg.L-1), and significant reduction of estrogenic activity, confirmed by e-screenassay. In vivo assays were performed in Erlennmeyer flasks on a rotary shaker at 120 rpm containing: medium PDB, solution of Bisphenol-A and fungal inoculum, for 54 hours cultivation and removal of aliquots every six hours. In in vitro experiments was added a solution of Bisphenol - A to 72 hours old cultive and maintained for 24 hours, removing aliquots every six hours. HPLC Chromatographic analysis showed that there was no significant mycelial adsorption. The white rot fungi Trametes sp . and Pleurotusostreatus have potential for the removal of Bisphenol - A, reducing significantly its activity estrogenic.
Biography
Alexandre Nunes Ponezi completed his PhD in Sanitation and Environmental and researcher at Multidisciplinary Center for Chemical, Biological and Agricultural from State University of Campinas (CPQBA/UNICAMP). He is environmental manager and professor in PhD program from State University of Campinas - Brazil; and has been serving as a reviewer for international journals.
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