alexa Restriction Of Heterologous Lentiviruses By Caprine Trim5α
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

5th World Congress on Biotechnology
June 25-27, 2014 Valencia Conference Centre, Valencia, Spain

L. de Pablo, P. J?uregui, L.Sanjos?, H.Crespo, I. Glaria, D. de Andr?s, B. Amorena and R. Reina
ScientificTracks Abstracts: J Biotechnol Biomater
DOI: 10.4172/2155-952X.S1.027
Abstract
Trim5α (T5α) is an important antiretroviral element of the innate immune response and plays an important role in species- specific restriction of lentiviruses by docking viral capsid protein and leading itto proteasome-mediated digestion. Over expression of ovine T5α appears to be responsible for restriction against Visna/ Maedivirus (VMV), as described in our previous studies. In this study we explore the restriction pattern of caprinecells (belonging to a goat synovial membrane cell line, GSM-T) against a panel of vesicular stomatitis virus protein G (VSV-G) pseudotyped heterologous retroviruses HIV-1, HIV-2, SIVmac, MLV-N and MLV-B. Upon exposure of GSM-Tcells to the pseudotyped viruses, SIVmac and MLV-N showed significantly decreased titers compared to those found in permissive feline cells (CRFK) used as control, which suggested the existence of a restriction factor in the GSM-T cell line.To determine its existence, T5α from GSM-T ( Ca6 )was cloned into EXN plasmid and the resulting vector packaged into VSV-Gpseudotyped MLV cores, yielding virionsen coding Ca6. MDTF cells transduced with these virions stably expressed Ca6and effectively restricted incoming SIVmac particles but not those of MLV-N. Therefore, thecaprineT5αCa6appeared to be responsible for the restriction of SIVmac observed. However, additional molecules (other T5isoforms or restriction factors like APOBEC3 or Tetherin) are needed to explain restriction against MLV-N.Lentiviral restriction by innate immunity intrinsic factors of heterologous origin may open new therapeutic strategies against human and animal lentiviral infections.
Biography
L. de Pablo is recipient of a pre-doctoral fellowship of the Public University of Navarre (Pamplona Spain) at the Institute of Agrobiotechnology (CSIC-UPNA- Government of Navarre). Her research on animal health is carried out in the fields of immunology, genetics and virology. Her thesis entitled ?Innate immunity against small ruminant lentiviruses (SRLV)? involves the study of retroviral restriction factors. Lorena de Pablo (Logro?o, 1986) graduated in Biology, obtained her Master?s degree in Health Sciences at the University of Navarre (Pamplona, Spain) and has different Congress contributions. Presently, she is working in the experimental phase of her pre-doctoral period, being enrolled at the Public University of Navarre doctoral study program.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords