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Screening For Novel Clot Busters From Food And Environmental Samples | 4719
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
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Screening for novel clot busters from food and environmental samples

3rd World Congress on Biotechnology

Vaibhav Sharma, Dipti Sinha and C. Subathra Devi

Posters: Agrotechnol

DOI: 10.4172/2155-952X.S1.020

Abstract
Clot busting is the breakdown or lysis of blood clots by pharmacological means and is colloquially referred to as thrombolysis. Thrombolysis suggests the use of thrombolytic agents, which can be derived from certain microorganisms. Here we are using Staphylococcus spp. to produce Staphylokinase (SAK), which is used to activate plasminogen. It functions by stimulating fibrinolysis by activating plasminogen to form plasmin through the infusion of analogs of tissue plasminogen activator (tpa) and can break up clots which may block the blood flow to the heart muscle. Staphylokinase (SAK) is a bacterial kinase produced by certain strains of Staphylococcus spp. It is a 15.5 kDa, consisting of 136 amino acid single chain protein, which activates plasminogen to form plasmin and digest fibrin clots. The present research work was carried out to screen the staphylokinase producing Staphylococcus spp. from different environmental and food samples collected (milk, water and sewage) in and around Vellore district, Tamil Nadu, India. Out of 12 isolates, 4 organisms were producing staphylokinase effectively.
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