alexa

GET THE APP

Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Screening Of Maize Germplasm For CrtRB1 Polymorphism And Association With HPLC Profiling For Increased ?-carotene Content | 4628
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 1820

Journal of Biotechnology & Biomaterials received 1820 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Screening of maize germplasm for crtRB1 polymorphism and association with HPLC profiling for increased ?-carotene content

3rd World Congress on Biotechnology

Dhyaneswaran Palanichamy, N.Senthil, M.Nivedhita, A. Mahalingam, John Joe, A. Yuvarajand, A. Aysha, D.Thirusendura Selvi, P. Prabha, P. Srimathi, P. Nagarajan and M. Raveendran

Posters: Agrotechnol

DOI: 10.4172/2155-952X.S1.020

Abstract
Biofortification of maize is an inexpensive and locally adaptable method to alleviate Vitamin A deficiency which is one of the major health problems in the world. Maize displays considerable natural variation for carotenoid composition, including vitamin A precursors α-carotene, β-carotene, and β -cryptoxanthin. The gene encoding β--carotene hydroxylase 1 (crtRB1) underlies a principal quantitative trait locus associated with β--carotene concentration and conversion in maize kernels. 316 maize inbred lines that were obtained from CIMMYT (International Maize and Wheat Improvement Center), Mexico and Directorate of Maize Research, New Delhi were grown and evaluated for crtRB1 polymorphism using gene specific markers. In order to detect the crtRB1 polymorphism the primer HYDB 3?TE was used. The presence of 543 bp (Allele size 1) indicates high, 296+1221+1800 bp (Allele size 3) indicates medium and 296+875bp (Allele size 2) indicates lower level of β-Carotene in maize. DMR30 and DMR38 were recorded withallele size 1. Allele size 2 was recorded inDMR35, DMR16 andDMR254. Allele size 3 was recorded in DMR99, DMR102, DMR103, DMR112, DMR113, DMR104, DMR231, DMR26 and DMR19. The identified genotypes with favorable alleles for crtRBI polymorphism were evaluated for whole kernel carotenoids using spectrophotometer analysis and β-carotene content was evaluated using high performance liquid chromatography (HPLC). The total carotenoid content rages from10.17 μg/g to29.1 μg/g. The beta carotene content ranges from 0.33 μg/g to 9.27 μg/g. Medium level of β carotene was observed in DMR 26 (4.009362 μg/g), DMR 112 (2.009941 μg/g). Higher level was observed in DMR 104 (9.278696 μg/g) and DMR 38 (7.076111 μg/g). Lower level of beta carotene content was observed in DMR 35 (0.330467 μg/g), DMR 102 (0.277442 μg/g), DMR 103 (0.361596 μg/g), DMR 113 (0.810067 μg/g). In PCR assay the 543-bp allele of crtRB1 leading to higher β carotene concentrations, was detected only in lower frequency (1.03%). This study shows that the selection of maize inbreds with enhanced seed beta carotene content is possible by selecting for crtRB1 allele.
Biography
Dhyaneswaran Palanichamy is a master?s student in Biotechnology and Business Management at Tamil Nadu Agricultural University (TNAU), Coimbatore. He earned his bachelor?s degree in Biotechnology in TNAU and Masters of Professional Studies in Plant Breeding degree from Cornell University, NY, USA. He is about to pursue his PhD in Plant Breeding at Cornell University starting from fall, 2012.
https://milanobet.fun/ https://mobilbahis.fun/ https://oleybet.fun/ https://onbahis.fun/ https://padisahbet.fun/ https://piabetgiris.xyz/ https://pusulabet.fun/ https://pulibet.fun/ https://restbet.fun/ https://satbet.fun/ https://1xbetx.fun/ https://asyabahisx.xyz/
Top