Journal of Biotechnology & Biomaterials
Open Access

Abstract

Ganoderic acid is a triterpenoid, anticancer produced by Ganoderma lucidum. In the present research the Plackett- Burman experimental design was used to identify the important medium constituent influencing the production of ganoderic acid B by Ganoderma lucidum NRCM OE 52 under submerged fermentation. Nine medium parameters such as maltose, glucose, corn flour, MnSO4.H2O, urea, soya flour, NaCl, biotin and Penicillium citrinum extract as elicitor were screened by Plackett ? Burman Design in 12 experimental run which include 9 medium parameters and 2 dummy variables. Nine nutrient parameter were maltose, glucose, corn flour, MnSO4.H2O, urea, soya flour, NaCl, biotin and elicitor shows 1.80 %, 0.96 %, 10.56 %, 0.84 %, 30.06 %, 10.71 %, 30.02 %, 1.86 %, 1.055 % respectively towards ganoderic acid B production by Ganoderma lucidum under submerged fermentation. Fermentation was carried out under shake flask condition with 10% v/v seed culture and incubated for 4 days under shaking conditions and under static condition for 8 d at temperature 27 0C. Among nine nutrient parameters urea and NaCl contributed maximum while corn flour and soya bean contributed moderately and MnSO4.H2O, maltose, glucose, biotin and elicitors had little impact on production of ganoderic acid B by Ganoderma lucidum.

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