Journal of Biotechnology & Biomaterials
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
The completion of human genome project resulted in opening of new vistas for understanding the gene expression. A number of specialized fields of studies, the ?omics? have been initiated. The proteomics is one of these branches that deals with the analysis of protein profile of a cell or tissue under different physiological condition. All the metabolic activities of cell are regulated directly or indirectly by proteins. The analysis of proteins, therefore, gives better insight into the metabolic status of cell than studying its genomics. This approach is now being used for discovering novel biomarker(s) that can be used to diagnose,
predict the susceptibility and monitor progression of diseases. Direct analysis of serum or other biological fluids is one of the most convenient strategies for the search of protein biomarkers. Hepatocellular carcinoma (HCC) is an international problem. It is the
third most prevalent cancer and the fifth leading cause of cancer deaths worldwide (WHO-2008). The current standard diagnosis
of HCC is based on the detection of serum α-fetoprotein (AFP) level. However, AFP has relatively low sensitivity (64.8% - 78%)
and low specificity (50% - 93%). More reliable and accurate biomarkers are urgently needed to overcome the shortcoming of the current tools for HCC diagnosis. We report the development of an animal model for study of HCC by modifying the Solt ?Farber protocol. The protein profiles of serum have been analysed by 1D SDS PAGE and 2D electrophoresis. Few specific changes in
proteins profiles have been detected that bear correlation with disease progression. Marker enzymes have also been monitored
to decipher the disease condition. Histopathology of liver tissue was performed to confirm liver damage. Differentially expressed
proteins and the novel proteins that are expressed during tumor progression have been characterized by MALDI-TOF and LC-
MS/MS techniques. The expressions of genes for the proteins of interest are in progress in our laboratory. Further, patient sera
from clinically confirmed cases have been used to validate these markers. Our results suggest that some of these proteins have
showing potential for the development of biomarker(s) for early detection of HCC.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals