Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
GET THE APP
Serum Glucose And Serum Protein Levels In Plasmodium Falciparum Infected Experimental C57BL/6J Mice | 4647
Journal of Biotechnology & Biomaterials
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Background: Malaria remains the most important parasitic disease afflicting about 2.2 billion people globally. Most malaria deaths are due to Plasmodium falciparum infection. P. f is an intra erythrocytic protozoan parasite. It exports antigens and imports extra cellular nutrients to survive. Serum albumin and its associated fatty acids are essential for intra erythrocytic development and cell cycle progression of the P. falciparum. A change in serum glucose level & protein level is a characteristic feature of much parasitic infection.
Materials and Methods: 12 week old C57BL/6J mice were divided into 6 batches each with 6 mice. These experimental groups were Control (Batch-1), Infected (Batch-2), Infected+Arteether treated (Batch-3), Infected+Artesunate treated (Batch-4),
Arteether Prophylactic (Batch-5) and Artesunate Prophylactic mice (Batch-6). Serum Glucose levels were determined by Ortho-toluidine method (Mono-step). Serum proteins levels were determined by Biuret and BCG (Bromo Cresol Green) Dye Binding method. Statistical analysis such as Mean, Standard Deviation (SD), Standard Error of Mean (SEM) and Paired T-Test were
performed with ?MINITAB 11. 1232 Bit?. For between group comparisons ?Two Sample T-Test? was performed. Differences were considered statistically significant at p<0.05 and p<0.001.
Results and Discussion: In the present investigation glucose levels were significantly decreased (p<0.001) causing hypoglycemia
at high rate of parasitemia in P. f infected mice. At high rate of parasitaemia malaria parasite utilizes glucose for theirs own
energy requirement. And malaria fever and infections increase the metabolic demand of the body hence more glucose is utilized.
When these infected mice treated with Arteether/Artesunate the glucose levels were increased significantly (p<0.001) to normal
level. In mice treated with Arteether/ Artesunate prophylaxis the blood glucose levels were significantly high (P<0.001) when
compared with infected mice, but these levels were in normal as in control group. And also low serum total protein concentration
was observed in this study may be due to reduced concentration of albumin i.e., hypoalbuminea. Hence the serum proteins, serum albumin levels were found to be significantly lower (p<0.05) compared to control group. Total globulins have increased significantly (p<0.05) due to P. f infection. When the P. f infected mice treated with Arteether/ Artesunate and during prophylactic treatment; total serum proteins, total albumin and total globulin levels restored to normal values. Also the finding suggested that
the level of total globulins is influenced by the degree of parasitemia. It is said that the high levels of globulins are due to acute or
chronic infection of P. falciparum.
Conclusion: By evaluating the above parameters in treated mice; Artesunate and Arteether have proven as still efficient drugs to
human malaria, Plasmodium falciparum when compared with other existing drugs in spite of increasing drug resistance.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals