Shotgun Proteomic Analysis Of Ascorbate-primed And Unprimed Wheat Seeds During Germination Under Salinity Conditions | 17281
Journal of Biotechnology & Biomaterials
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Seed priming with antioxidant compounds has been successfully employed to improve salt tolerance in crop plants. For
understanding the potential mechanisms underlying this priming effect a gel-free shotgun proteomic analysis was performed
comparing between unprimed and ascorbate-primed wheat seeds during germination under saline and non-saline conditions.
of 697 identified proteins 167 were significantly up- or down-regulated in response to priming and/or salinity compared to
untreated, unstressed control. In untreated wheat embryo salt stress was accompanied by change in 129 proteins, most of
which are involved in metabolism, energy, disease/defense, protein destination and storage functions. Ascorbate pretreatment
prevents and counteracts the effects of salinity upon most of these proteins and changes specifically the abundance of 35 others
proteins, most of which belonging to metabolism, protein destination and storage categories. Hierarchical clustering analysis
revealed three major clusters of protein expression. These results may provide new insight into the molecular mechanisms
underlying priming-induced salt tolerance in plants.
Azzedine Fercha is a lecturer in plant biochemistry at Abbes Laghrour University, Khenchela, Algeria. He graduates in plant physiology and biochemistry at
Constantine-I University, Algeria. During his training he moved to La sapienzauniversit? di-Roma, Italy, to exploit proteomics approaches for studying plant
responses to abiotic stresses. He works as associate researcher in many research projects. Most of his research works have been focused on the physiological
and molecular responses of crop plants to abiotic stresses, particularly to salt stress. He is co-author of more than 10 papers in international peer-reviewed journa
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