Journal of Biotechnology & Biomaterials
Open Access

Abstract

The improvement on insulin therapy since it was first administered in early of 20th century is still hunting for better option to cure the diabetes. But insulin therapy to the patients for long periods resulted in immunological complications due to the development of insulin antibodies that cause insulin allergy and many chronic disorders. The adverse effects and higher cost of insulin had compelled scientists all over world to look for plant based insulin. Since its discovery, a formidable amount of research, strongly suggest that plants produce a protein with most of the characteristics of vertebrate insulins and plays similar roles in plants as it does in animals. There is growing interest throughout the world in Spirulina as a potential source of nutraceutical compounds, which have applications in health food, feeds, therapeutics and diagnostics. In this study, we screened different strains of Spirulina for the presence of insulin, surprisingly, most out of them showed presence of insulinlike protein. Electrophoretic mobility analysis, antigenicity test and RP-HPLC showed similar characteristic as that of bovine insulin. Secondary structure analysis of insulin-like protein by circular dichroism was also done and showed almost same �?± helical content. Besides this, the molecular dynamics simulations studies of insulin also suggesting an interesting result. It has two constant values at different time scales. The relative folding energy of insulin like protein from plant source suggested that it has maximum stability at neutral pH, i.e., similar to pH of human blood.

Biography

Email:

raziq_anwer@yahoo.co.in