Journal of Biotechnology & Biomaterials
Open Access

Abstract

In this study we tried to determine the biochemical composition and evaluate the antimicrobial activity of Spirulina platensis from one Algerian aquatic ecosystem. Biochemical substances extracted are primarily analyzed by colorimetric and chromatography (TLC and CPG) methods and then tested on a set of microorganisms widely encountered in humans. Analysis of the results has shown that this cyanobacterium is rich in matrix polysaccharides. Indeed, the dosage of polysaccharide extracts by the colorimetric method revealed a concentration 13, 15 mg / l for matrix polysaccharides against 9, 47 mg / l for fiber polysaccharides. Simple sugars disclosed by TLC are galactose, glucose, rhamnose and levrose. Regarding lipids, qualitative analysis found a richness of unsaturated fatty acids. The quantitative GC analysis shows that this cyanobacterium contains 39.018% of saturated fatty acids and 55, 277% of unsaturated fatty acids. As for proteins, the Bradford method assay shows a high concentration (1, 45 mg/ml). The spectral characterization and pigments chromatography highlighted the presence of carotenoids, chlorophyll A and B, xanthophyll, phycocyanin and phycoerythrin. Finally, the evaluation of the antimicrobial activity of polysaccharides, proteins, fatty acids and pigments indicated that polysaccharides, proteins and chlorophyll B have no effect on the microorganisms tested. However, the other extracts possess activity on bacteria tested while showing differences in their activity on Candida albicans. It is important to note that the only strain tested fungal (Aspergillus niger) has proven resistant to all the extracts used.

Biography

Email:

Yahiaoui_karima2005@yahoo.fr