Studies On Detection And Characterization Of Shigatoxigenic Escherichia Coli (STEC) And Enteropathogenic Escherichia Coli (EPEC) Associated With Diarrhoea In Infants In Mizoram | 4734
Journal of Biotechnology & Biomaterials
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Diarrhoeagenic Escherichia coli (STEC and EPEC) are responsible for an estimated 300,000-500,000 death annually in
children under the age of 5 years. STEC and EPEC are commonly recovered from the faeces of food producing animals
and pose threats to health of humans and livestock. A total of 264 faecal samples were collected from infants with the history of
diarrhoea from different hospitals of Mizoram. Samples were processed in laboratory for isolation of E. coli. All the E. coli isolates
were screened for STEC and EPEC encoding virulence genes by PCR assay. All the virulence gene carrying E. coli isolates were
subjected to serotyping (O & H), subtyping for stx
subtypes by PCR, detection of bfpA gene by PCR, vero cell cytotoxicity of
STEC isolates and antimicrobial sensitivity assay.
A total of 540 E. coli isolates were isolated from infants. All together 17 (3.15 %) E. coli isolates were found to be positive
for at least one virulence gene of which 8(1.5%) and 9 (1.67%) were recorded as STEC and EPEC, respectively. Of the total 8
positive isolates, 5 (62.5%) were positive for stx
c and 3(37.5%) were positive for stx
e subtypes, respectively. Of the
17 (3.15%) eaeA positive isolates 3(17.65%) were positive for bfpA gene. 2 different somatic types and 4 different flagellar types
were recorded. All the isolates were exhibited varying degree of CPE on vero cell lines. Enrofloxacin exhibited 100% sensitivity,
whereas amoxicillin, polymyxin B and kanamycin exhibited 100% resistance in infant isolates during antimicrobial sensitivity
assay. Serogroup O60 E. coli may be the emerging pathogens of infants in Mizoram.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals