Journal of Biotechnology & Biomaterials
Open Access

Abstract

Nanoparticles have emerged as one of the most promising drug delivery systems in the field of chemotherapy for localized and metastasized cancers owing to their unique potentials like high intracellular uptake, enhanced permeability and retention while reducing undesirable side effects. A number of naturally occurring polyphenols, present in most commonly consumed beverage tea, has been shown to possess anticancer properties but has associated with limited success including inefficient systemic delivery and bioavailability under in vivo conditions. Our present work investigated the efficiency of polyphenolic constituents of black (Theaflavin-TF) and green (epigallocatechins-3-gallate-EGCG) tea encapsulated in poly (lactic-co-glycolic acid) nanoparticles (NPs) using double emulsion method. The nanoparticles formulated were and then characterized for encapsulation efficiency, surface morphology, particle size and in vitro release. TEM analysis showed the formation of spherical and monodisperse nanoparticles, with an encapsulation efficiency of ~18% and 26% for TF and EGCG, respectively. Further their preventive potential against 7, 12-dimethylbenzanthracene (DMBA) induced DNA damage in mouse skin using DNA alkaline unwinding assay (DAUA) was evaluated. Pre-treatment (topically) of mouse skin with TF or EGCG (100μg/mouse) doses exhibits protection of 45.34% and 28.32%, respectively, against DMBA induced DNA damage. Additionally, TF/EGCG loaded NPs have showed significant potential for induction of DNA repair genes (XRCC1, XRCC1 and ERCC3) and suppression of DNA damage responsive genes (p53, p21, MDM2, GADD45α and COX-2) as compared to respective bulk doses. Hence, our studies achieved successful formulation of EGCG and TF loaded PLGA nanoparticles and has high potential to control DMBA induced DNA damage at much more reduced concentrations and thus opens a new arena for clinical application in prostate cancer therapy.

Biography

Priyanka Bhatnagar has completed her M.Sc. in Chemistry (Organic) from University of Delhi, India in 2009. She is pursuing her Ph.D in Bio-organic Chemistry from Institute of Genomics & Integrative Biology (IGIB), CSIR Lab, New Delhi, India. She is working in the area of design and development of Polymeric systems for Effective Drug Delivery. She has 1 paper in reputed journal.