The Antibacterial Evaluation Of Honey And Moringa (Moringa Oleifera) Leaf Extract Against Bacteria Isolated From Inanimate Surfaces Of A Hospital | 64941
Journal of Infectious Diseases & Therapy
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Statement of Problem: Healthcare facilities today harms too frequently and often fails to deliver its potential benefits because
pathogenic microorganisms are readily present on fomites surfaces, thereby increasing the spread of nosocomial infections. The aim
of this study was to investigate the types of microorganisms associated with selected fomites surfaces of the female and male wards of
the FUTA Health Centre, Akure, Ondo state of Nigeria.
Methodology: A total of 28 samples were collected from the bed linens, bed rails, bedside cabinets, door knobs, chairs, floor, wall and
sink faucets from the male and female wards using sterile swab sticks moistened with normal saline and cultured on different selected
media. The antibacterial efficacy of ethanol extract of Moringa oleifera (Lam) leaf and pure honey were investigated in vitro against
the isolated bacteria using the agar well diffusion technique at different concentrations.
Findings: The bacteria isolated were Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus cereus,
Staphylococcus epidermidis, Micrococcus spp., Enterococcus spp., and Escherichia coli in descending order of prevalence. All the
bacterial isolates were successfully inhibited by Moringa oleifera at all concentrations. The zone of inhibition increased with increase
in the concentration of the leaf extract. All the bacteria isolates were inhibited by pure honey at 100% concentration with the highest
zone of inhibition of 20.5 mm recorded for Staphylococcus aureus. At 75% concentration, Staphylococcus aureus, Enterococcus spp.,
and Klebsiella pneumoniae were the only bacteria inhibited. At 25% and 50% concentrations, no bacterium was inhibited. This result
showed that Moringa oleifera leaf extract and pure honey exhibited a dose-dependent effect on the bacterial isolates and that inanimate
surface of hospital environment houses varieties of bacteria may be responsible for nosocomial infections.
Omoya F Oluyemi is a Senior Lecturer and Researcher. She has her expertise in Environmental Health and Bio-control. She has researched on several infections that are caused mainly by poverty and environmental hazards, investigating in vitro effect of different plants extracts and honey on the etiologic agents.