The Application Of Combinatorial Biotechnology To Crop Improvement: A Case Study On Potato | 17057
Journal of Biotechnology & Biomaterials
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The new tools of plant biotechnology can bypass sexual incompatibility in order to improve plant resistance to biotic and
abiotic stress. Today, when many plant genomes have been already sequenced and many molecular tools are available,
combining more tools, or combinatorial bitechnology, towards a sustainable crop improvement becomes more feasible. Potato
as a very important crop ranking third at global level in productivity is a good example for biotechnological improvement.
The great majority of the wild relatives of the genus
growing in Central and South America carry many genes of
resistance but are sexually incompatible with the tetraploid potato crop. Examples on combining cell fusion technique with
molecular assisted selection (MAS), genetic manipulation of DNA mismatch repair system (MMR), different bioassays to
discriminate ways of resistance to Colorado potato beetle (CPB) such as repellence or toxicity, the involvement of natural
resistance mechanisms through specific glycoalkaloids (leptines) or trichomes analysis are going to be presented for the
combination potato +
. Other examples will involve multiple resistance gene transfer to potato from the
wild species S. cardiophyllum or S.
. A particular case to be detailed is the fusion hybrids between potato varieties and S.
, where gene specific markers, resistance assessments in the laboratory and a field give very good results in the
transfer of resistance to late blight, the most important disease of potato.
Elena Rakosy-Tican is a Professor at Babe Bolyai University, Romania from 2008 in Plant Genetic Engineering Group, Faculty of Biology and Geology, Babes-
Bolyai University Cluj-Napoca Romania. His area of expertise is plant genetic manipulation. She was awarded her PhD in Biology/Genetics in Jan 1994 from Babe
Bolyai University, Romania.
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