The Association Of Single Nucleotide Polymorphism Of Interleukin-21 Gene And Serum Interleukin-21 Levels With Systemic Lupus Erythematosus | 53206
Journal of Biotechnology & Biomaterials
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Background: Systemic lupus erythematosus (SLE) is a common autoimmune disorder which commonly results from the
combined effects of a large number of genes. Variations in the DNA sequence in the Interleukin-21 (IL-21) gene may lead to
altered IL-21 production and/or activity which can affect an individual’s susceptibility to SLE. IL-21 is a novel class I cytokine
produced by activated CD4+ T cells, natural killer T cells and T helper (Th) cells. There is increasing evidence that IL-21
contributes to the pathogenesis of SLE due to its biological activity.
Aim: To investigate the association between single nucleotide polymorphism (SNP) of IL-21 rs2221903 gene and serum IL-21
levels with SLE and to detect the possible association between IL-21 serum levels and the pathogenesis of the disease.
Subjects & Methods: This study was conducted on 30 SLE patients and 20 age and sex matched healthy controls. Serum IL-21
levels were measured using enzyme-linked immunosorbent assay (ELISA) technique and SNP of IL-21 rs2221903 gene was
detected by genotyping assay, using real-time polymerase chain reaction (RT-PCR).
Results: Serum IL-21 levels were significantly higher in patients compared with controls (p<0.001). Patients with high activity
index of SLE had significantly higher levels of serumIL-21 (p value<0.001). A statistically significant association was found
between the T allele of SNP rs2221903 and SLE, whereas; no association between SNP of IL-21 rs2221903 genotypes and SLE
or serum IL-21 levels could be detected.
Conclusion: IL-21 plays an important role in the immune-pathogenesis of SLE and could be used as a possible target for novel
immunotherapy. The T allele of SNP rs2221903 suggests that the IL-21gene may contribute to an inherited predisposition to
Dina Mohammmad Erfan has completed her MD and Postdoctoral studies from Faculty of Medicine, Ain Shams University, Egypt.