The Influence Of Seasons And Ripening Time On Yeast Communities Of A Traditional Brazilian Cheese | 102640
Journal of Biotechnology & Biomaterials
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Traditional Minas cheese is an artisanal cheese manufactured by farmers on a small scale, utilizing raw bovine milk. The
occurrence and effects of the dry and rainy seasons on yeast populations in traditional Serro Minas cheese, one of the most
popular cheeses produced in Brazil, were studied over the course of 60 days of ripening. A total of 19 yeast species were identified
via sequence analysis of the D1/D2 domains of the large subunit of the rRNA gene. Fourteen yeast species were obtained from
cheese produced during the dry season and fifteen species were obtained from cheese produced during the rainy season. High
diversity indices for the yeast species were determined for cheese manufactured during both seasons (average H???D=1.7 and
H???R=1.5, respectively). The predominant species in Serro Minas cheese included Debaryomyces hansenii, Kodamaea ohmeri
and Kluyveromyces marxianus. Debaryomyces hansenii 28.12 showed low lipolytic and high proteolytic activity. Kluyveromyces
marxianus 83F and 60P demonstrated lipolytic and ?-galactosidase activity, respectively. Kodamaea ohmeri 88A displayed low
lipolytic and ?-galactosidase activity. Maximal lipase, ?-galactosidase and protease activity were observed at 20°C and pH 6.0,
30°C and pH 7.0 and 50°C and pH 6.0, respectively. Considering that D. hansenii 28.12, K. ohmeri 88A and K marxianus 60P
together showed protease, lipase and ?-galactosidase activity in this study, further research on the possibility of including these
yeasts as part of a starter culture and research on their effects on the sensory properties of Serro Minas cheese merit more study.
Valeria Macedo Cardoso is a Pharmaceutical, with Master in Pharmaceutical Science and PhD in Food Microbiology (2011) from the Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. She is a permanent Professor in the Pharmacy Department, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Minas Gerais, Brazil. Her research focuses on the area of food microbiology and food quality issues.