Toxicological Evaluation Of Chlorpyrifos, Cypermethrin And Their Combination In Earthworm And Their Impact On Acetylcholinesterase | 106709
ISSN: 2476-2067

Toxicology: Open Access
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Toxicological evaluation of chlorpyrifos, cypermethrin and their combination in earthworm and their impact on acetylcholinesterase

20th World Congress on Toxicology and Pharmacology

Rishikesh Kumar Tiwari, Shikha Singh and Ravi S Pandey

University of Allahabad, India

ScientificTracks Abstracts: Toxicol Open Access

The increasing applications of pesticides in the agricultural fields have adverse impact on flora and fauna of the soil ecosystem. The role of earthworms in the agricultural practices is well known as they immensely contribute in increasing the quality and fertility of soil. So, it acts as a bio-indicator for the ecotoxicological analysis of pesticide induced soil pollution. Therefore, the present study was aimed to explore the impact of chlorpyrifos (an organophosphate; OP), cypermethrin (a pyrethroid) and their combination (chlorpyrifos+cypermethrin) on earthworm, Eudrilus eugeniae. E. eugeniae were exposed to different concentrations of pesticides for 48 hours by paper contact toxicity method. The LC50 for commercial grade chlorpyrifos, cypermethrin and their combination were determined as 0.165, 0.020 and 0.066 ?g/cm2 respectively. To assess the sub-lethal effect of these pesticides, E. eugeniae were exposed to 5% and 10% of LC50 pesticides for 48 hours. Alterations in morpho-behavioral patterns such as coiling, clitellar swelling, mucus release and bleeding followed by fragmentation of body in earthworms were observed following exposure. Acetylcholinesterase (AChE) activity was assayed in different regions of body segment which exhibits significant (p<0.05) decrease in AChE activity particularly in pre-clitellar region followed by clitellar and post-clitellar regions and in comparison, to whole body. The decreased AChE activity with increasing concentration of pesticides indicates the effect at neuronal level which apparent from the behavioral changes. Therefore, from the present findings it can be concluded that long term exposure to these pesticides could lead to severe and irreparable effects on biochemical mechanisms of earthworms.

Rishikesh Kumar Tiwari is presently working as a Doctoral Fellow with Professor Ravi S Pandey, Biochemistry Laboratory, Department of Zoology, University of Allahabad. He has completed his MSc with Cytogenetics specialization from University of Allahabad, India. He has 10 research/review articles in national/ international journal of repute and one book chapter in USA based publishing house.

E-mail: [email protected]

[email protected]