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Transformation Of Exogenic Anti-microbial Regulon From Ethnomedicinal Plant Against Crop Pathogens By Iterative Genetic Transformation Approach | 8524
ISSN: 2155-952X
Journal of Biotechnology & Biomaterials
Open Access
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My first objective in creating exogenous antimicrobial
regulon is to identify the potential antimicrobial
key biomolecule from the ethnomedicinal
plant. A set of three plants used by tribal healers to treat
infections, were screened for antibacterial properties at
different concentration by using disc diffusion method
against pathogenic fungus and bacteria that affect most
important crops. The leaf extracts of Cassia occidentalis,
Datura metal and Clerodendron inerme exhibited
significant broad spectrum activity against the crop
pathogens. These results were compared with results
obtained using standard antibiotics, chloramphenicol
(30 mg: disc) and streptomycin (30 mg: disc) which
served as a reference for inhibition zone diameter. The
Qualitative analysis is very essential for identifying
the compounds present in these plants. We have done
quantitative analysis for these plants. The results are
discussed in this paper. Our further studies include
determination of the metabolic pathway for the
synthesis of the key active compound responsible for the
antimicrobial activity. Next step is to iterating the process
of genetic transformation of the genes that synthesise the
enzymes involving in the biological synthesis of the key
active compound with each gene construct having same
regulatory sequences under a constitutive promoter.
This results in inserting exogenous anti-microbial
regulon in vitro by genetic engineering.
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