Journal of Biotechnology & Biomaterials
Open Access

Abstract

My first objective in creating exogenous antimicrobial regulon is to identify the potential antimicrobial key biomolecule from the ethnomedicinal plant. A set of three plants used by tribal healers to treat infections, were screened for antibacterial properties at different concentration by using disc diffusion method against pathogenic fungus and bacteria that affect most important crops. The leaf extracts of Cassia occidentalis, Datura metal and Clerodendron inerme exhibited significant broad spectrum activity against the crop pathogens. These results were compared with results obtained using standard antibiotics, chloramphenicol (30 mg: disc) and streptomycin (30 mg: disc) which served as a reference for inhibition zone diameter. The Qualitative analysis is very essential for identifying the compounds present in these plants. We have done quantitative analysis for these plants. The results are discussed in this paper. Our further studies include determination of the metabolic pathway for the synthesis of the key active compound responsible for the antimicrobial activity. Next step is to iterating the process of genetic transformation of the genes that synthesise the enzymes involving in the biological synthesis of the key active compound with each gene construct having same regulatory sequences under a constitutive promoter. This results in inserting exogenous anti-microbial regulon in vitro by genetic engineering.

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