Journal of Infectious Diseases & Therapy
Open Access

Abstract

Background: Influenza viruses, respiratory-syncytial virus (RSV), metapneumovirus (hMPV), parainfluenza viruses (hPIV)
type 1, 2 and 3 are leading causes of acute respiratory tract infections (ARTI) in infants and young children. The aim of this
study was to determine the contribution of these viruses in medically attended ARTI among children aged <4 years during the
2012/13, 2013/14 and 2014/2015 winter seasons in Bulgaria.
Methods: A total of 555 nasopharyngeal swabs of children aged <4 years diagnosed with ARTI were collected and tested for
influenza A/B viruses by Real Time RT-PCR. Influenza virus negative samples were examined by individual Real Time RTPCR
using specific primers/probes for RSV, hMPV, hPIV1/2/3.
Results: Of the 555 specimens tested 152 (27%) were influenza virus positive. Influenza A (H1N1) pdm09, A(H3N2) and
type B viruses were found in 64 (12%), 33 (6%) and 50 (9%) of samples, respectively. Among the 403 influenza virus negative
specimens, paramyxoviruses RSV, hMPV, hPIV1/2/3 were detected in 86 (21%), 28 (7%), 23 (5, 7%), 7 (1, 7%) and 20 (5%)
samples, respectively. RSV was the most frequently identified paramyxovirus. Overall, 33 (6%) patients were co-infected
with two viruses. The contribution of 8 examined respiratory viruses in cases of bronchiolitis, pneumonia and neurological
complications was analyzed.
Conclusions: During the last three winter seasons, influenza viruses and RSV were the most frequent viral pathogens causing
medically attended ARTI among children <4 years of age. Rapid diagnostics by Real Time RT-PCR is decisive in terms of
adequate treatment and taking prompt anti-epidemic measures.

Biography