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Copper is a very common element and distributing in all line things. In the mammals, the liver is the key organ to the copper homeostasis. I am interested in whether the disrupted copper homeostasis induces the changes in the liver cell. We pay attention to the changes of reactive oxygen species and the autophagy in the liver. My second interest is how the autophagy defection modifies the lipid and glycogen metabolism in the liver. We are going to utilize the autophagy gene knockout mice to test our proposals.