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Cloning and Analysis of N-Acetyltransferase 9 Genes in Yellow Catfish Pelteobagrus fulvidraco | OMICS International | Abstract
ISSN: 2168-9652

Biochemistry & Physiology: Open Access
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Research Article

Cloning and Analysis of N-Acetyltransferase 9 Genes in Yellow Catfish Pelteobagrus fulvidraco

Ming-Ming Han1,2, Jian-Guo Lu1, Le-Wang1, Li-Na-Peng1, Shahidd Mahboob3,4, Khalid A Al-Ghanim4 and Xiao-Wen Sun1*
1Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China
2Shanghai Ocean University, College of Fisheries and Life Science, Shanghai, China
3Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
4Department of Zoology, GC University, Faisalabad, Pakistan
*Corresponding Author : Xiao-Wen Sun
Heilongjiang River Fisheries Research Institute
Chinese Academy of Fishery Sciences, Harbin, China
Tel: +86-451-84869349
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E-mail: [email protected]
Received: February 13, 2016 Accepted: March 03, 2016 Published: March 10, 2016
Citation: Han MM, Lu JG, Wang L, Peng LN, Mahboob S, et al. (2016) Cloning and Analysis of N-Acetyltransferase 9 Genes in Yellow Catfish Pelteobagrus fulvidraco. Biochem Physiol 5:202. doi: 10.4172/2168-9652.1000202
Copyright: © 2016 Han MM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

N-acetyltransferase 9 (NAT9) is an important reproduction-related gene in fish. In this study, we cloned the fulllength NAT9 cDNA sequence from yellow catfish Pelteobagrus fulvidraco. P. fulvidraco NAT9 cDNA is 1253 bp, which including non-coding region (584 bp) and open reading frame (669 bp). NAT9 gene encodes a 222 amino acidsprotein which shares high homology with NAT9 in four species: Ietalurus punetaus (91%), Astyanax mexicanus (83%), Danio rerio (81%) and Lepisosteus oculatus (81%). NAT9 protein is 25.7899 kD, 4.86 theoretical isoelectric point and C1124H1759N311O359S13 as revealed by computer-assisted analysis. Tissue transcription profile analysis indicated that the catfish NAT9 gene is generally but differentially expressed in the detected tissues, including gonads, brain, liver, kidney, spleen, intestine, blood, gills, muscle and heart. Identification and tissue distribution of yellow catfish NAT9 genes provided initial step towards understanding their biological roles in yellow catfish.

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