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<em>Insilico</em> Analysis of cis acting Regulatory Elements CAREs in Upstream Regions of Ascorbate Glutathione Pathway Genes from <em>Oryza sativa</em> | Abstract
ISSN: 2168-9652

Biochemistry & Physiology: Open Access
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Research Article

Insilico Analysis of cis acting Regulatory Elements CAREs in Upstream Regions of Ascorbate Glutathione Pathway Genes from Oryza sativa

Saurabh Pandey1,2*, Chinreddy Subramanaym Reddy1, Ubaid Yaqoob3, Yogesh Kumar Negi4, Sandeep Arora5 and Tanushri Kaul1
1Plant Molecular Biology Lab, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067, India
2Uttarakhand Technical University, Dehradun-248007, India
3Department of Botany, University of Kashmir, Srinagar-190006, India
4Department of Basic Sciences, College of Forestry & Hill Agriculture, University of Horticulture & Forestry, Ranichauri, Uttarakhand-249199, India
5Department of Molecular Biology and Genetic Engineering, C.B.S.H, G.B.P.U, A & T, Pantnagar, Uttarakahand-263145, India
Corresponding Author : Saurabh Pandey
Plant Molecular Biology Lab
International Centre for Genetic Engineering and Biotechnology
Aruna Asaf Ali Marg, New Delhi-110067, India
Tel: +918447869135
Fax: +91-11-26742316
E-mail: [email protected]
Received March 18, 2015; Accepted April 22, 2015; Published April 29, 2015
Citation: Pandey S, Subramanaym Reddy C, Yaqoob U, Negi YK, Arora S, et al. (2015) Insilico Analysis of cis acting Regulatory Elements CAREs in Upstream Regions of Ascorbate Glutathione Pathway Genes from Oryza sativa. Biochem Physiol 4:159. doi:10.4172/2168-9652.1000159
Copyright: © 2015 Pandey S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

CAREs play an important role in plant stress tolerance by interacting with transcription factors and controlling the expression of many stress related potential genes. Recognition of promoters and their regulatory elements is one of the crucial challenges in biotechnology. In this study, we examine cis acting regulatory element in 5’ upstream regions (~1 kb) of Asc-Glu pathway genes such as SOD, APX, MDHAR, DHAR, and GR. The evolutionary relationships amongst these sequences were deciphered using MEGA v. 6.0. The promoter region these genes contain various cis acting regulatory elements such as MBS, DRE/C repeat, W box, HSE, TCA element, LTR, ABRE box, ARE box, Wun and DRE that have significant role in stress tolerance Asc-Glu promoter sequences analysis revealed their specific responsiveness or overlapping in various environment stress and significantly contribute toward plant growth and development.

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