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Microfibrillar-Associated Protein 4 (MFAP4) Genes in Pelteobagrus fulvidraco play a Novel Role in Innate Immune Response | OMICS International | Abstract
ISSN: 2168-9652

Biochemistry & Physiology: Open Access
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Research Article

Microfibrillar-Associated Protein 4 (MFAP4) Genes in Pelteobagrus fulvidraco play a Novel Role in Innate Immune Response

Ming-Ming Han1,2, Le-Wang1, Li-Na-Peng1, Shahidd Mahboob3,4, Khalid A Al-Ghanim4, Jian-Guo Lu1* and Xiao-Wen Sun1*
1Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China
2Shanghai Ocean University, College of Fisheries and Life Science, Shanghai, China
3Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
4Department of Zoology, GC University, Faisalabad, Pakistan
Corresponding Authors : Xiao-Wen Sun
Heilongjiang River Fisheries Research
Institute, Chinese Academy of Fishery Sciences
232 Hesong Road, Daoli District, Harbin 150070, China
Tel: +86-451-84869349
Fax: +86-451-84604803
E-mail: [email protected]
Jian-Guo Lu
Heilongjiang River Fisheries Research Institute
Chinese Academy of Fishery Sciences, 232 Hesong Road
Daoli District, Harbin 15007, China
Tel: +86- 451-84869349
Fax: +86-451-84604803
E-mail: [email protected]
Received: January 07, 2016; Accepted: February 05, 2016;Published: February 12, 2016
Citation: Han MM, Wang L, Peng LN, Mahboob S, Al-Ghanim KA, et al. (2016) Microfibrillar-Associated Protein 4 (MFAP4) Genes in Pelteobagrus fulvidraco play a Novel Role in Innate Immune Response. Biochem Physiol 5:198. doi:10.4172/2168-9652.1000198
Copyright: © 2016 Han MM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Both mannose-binding lectins (MBLs) and ficolins recognize and bind carbohydrates in pathogens and activate complement leading to opsonization, leukocyte activation, and direct pathogen clean. While MBLs have been reported in many fish species, the function of ficolins had not been identified in Pelteobagrus fulvidraco, despite their importance in invertebrate and higher vertebrate innate immunity. Although microfibrillar-associated protein 4 (MFAP4) this protein has similar fibrinogen-like domain, has expression in fish, the role of it has not been dissected. We cloned three MFAP4 genes (1MFAP4, 2MFAP4 and 3MFAP4) from the yellow catfish, P. fulvidraco. The homology similarity of MFAP4 was 78% with Astyanax mexicanus of Cypriniformes. The yellow catfish MFAP4 transcripts expression analysis revealed difference of patterns of homeostatic expression among the genes in gill, blood, muscle, gonad, liver, brain, spleen, kidney, heart, intestine from male and female, respectively. Expression of the three MFAP4 transcripts showed significant expression changes in 4 h after infection with either Edwardsiella ictaluri or Flavobacterium columnare, which has modulation of expression continuing up to 24 h or 7 d following pathogen exposure. Several different tissues and gene-specific patterns were captured and transcript expression changes of >11.45-fold were observed over the course of the bacterial challenges. This information elucidates the functions of individual MFAP4 genes in regards to pathogen recognition, binding, and their larger immune context.

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