Production and Characterization of Streptokinase Enzyme by Using Streptococcus mutans Strain in Liquid State Fermentation through Corn Steep Liquor (CSL) Substrate
|Abdul Ghaffar*#, Bilal Ahmed*#, Bushra Munir, Rana Faisal and Zahid Mahmood|
|Department of Applied Chemistry and Biochemistry, Main Allama Iqbal Road, Government College University, Faisalabad, Pakistan|
|#Authors contributed equally|
|Corresponding Authors :||Abdul Ghaffar
Department of Applied Chemistry and Biochemistry
Government College University, Faisalabad, Pakistan
Tel: +92 41 9200037
E-mail: [email protected]
Department of Applied Chemistry and Biochemistry Government College University
E-mail: [email protected]
|Received September 04, 2015; Accepted September 22, 2015; Published September 29, 2015|
|Citation: Ghaffar A, Ahmed B, Munir B, Faisal R, Mahmood Z (2015) Production and Characterization of Streptokinase Enzyme by Using Streptococcus mutans Strain in Liquid State Fermentation through Corn Steep Liquor (CSL) Substrate. Biochem Physiol 4:178. doi: 10.4172/2168-9652.1000178|
|Copyright: © 2015 Ghaffar A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Background: Enzymes play a very important and central role in the chemical reactions, occurring in biological systems. The study of enzymes has an immense practical importance. They have high catalytic power, many times greater than the synthetic catalysts. Vascular blockage can cause serious consequences leading to death by a thrombus (blood clot) developed in the circulatory system. Streptokinase is an extracellular protein Trans-located by many strains of beta-hemolytic Streptococci. By converting the plasminogen, to fibrin lytic enzyme, plasmin, it favor’s the blood clot lysis.
Results: The main objective of this research was the production of streptokinase in liquid state fermentation using CSL substances as substrate from Streptococcus species which was grown on blood agar media. Streptococcus mutans was selected for streptokinase production using corn steep liquor as substrate. Corn steep liquor (CSL) was applied in different concentrations ranging from 0.1% to 0.8% in liquid state fermentation culture medium and fibrin clot lysis method was used for enzyme assay.
Conclusion: Among different concentrations of CSL 0.3% was selected as optimum concentration, 44% blood lysis was observed by CSL in liquid state fermentation culture medium for streptokinase production.