alexa Protective Immunodominant Zaire Ebolavirus Glycoprotein
ISSN: 2157-2526

Journal of Bioterrorism & Biodefense
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Protective Immunodominant Zaire Ebolavirus Glycoprotein Epitope in Mice

Xiangguo Qiu1, Lisa Fernando1, Steven M. Jones1,2,3,4 and Judie B. Alimonti1,2*

1Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington St. Winnipeg, Manitoba, R3E 3R2, Canada

2Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, R3E 3R2, Canada

3Department of Immunology, University of Manitoba, Winnipeg, Manitoba, R3E 3R2, Canada

4Cognoveritas Consulting Inc. A-137 Westchester Dr., Winnipeg, Manitoba, R3P 2G6 Canada

*Corresponding Author:
Judie Alimonti
Special Pathogens Program, National Microbiology Laboratory
Public Health Agency of Canada, 1015 Arlington St.
Winnipeg, Manitoba, R3E 3R2
Tel: 204-784-5998 or 789-5097
Fax: 204- 789-2140
E-mail: [email protected]

Received Date: August 26, 2011; Accepted Date: October 18, 2011; Published Date: October 20, 2011

Citation: Qiu X, Fernando L, Jones SM, Alimonti JB (2011) Protective Immunodominant Zaire Ebolavirus Glycoprotein Epitope in Mice. J Bioterr Biodef S1:006. doi: 10.4172/2157-2526.S1-006

Copyright: © 2011 Qiu X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Zaire Ebolavirus (ZEBOV) causes a highly lethal severe haemorrhagic fever in humans, for which there is currently no approved vaccine available. In this study, cytokine ELISPOT assays were used to determine the ZEBOVGP-specific cytokine profile and immunodominant T cell epitopes in VSV?G/ZEBOVGP immunized Balb/C mice. Splenocytes added to pools of overlapping peptides spanning ZEBOVGP stimulated a Th1 cytokine profile with the majority of the ZEBOVGP-specific splenocytes secreting IFN-g. The splenocytes produced the strongest IFN-g response to the immunodominant peptide HNTPVYKLDISEATQ, located in the cytopathic mucin domain of GP. The immunodominant epitope was then tested for its ability to induce a protective immune response in mice challenged with a lethal dose of mouse adapted-ZEBOV. Mice immunized with the peptide plus Freund’s adjuvant survived the challenge, and had a strong ZEBOVGP-specific T and B cell immune response. Identifying the ZEBOVGP-specific cytokine profile and immunodominant epitope will aid in determining the correlates of immune protection; allow for the development of assays to assess the efficacy of the VSV?G/ZEBOVGP vaccine in immunized individuals; and provide valuable information for the development of a subunit vaccine.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords