Riboflavin (Vitamin B2) Assay by Adsorptive Cathodic Stripping Voltammetry (Adcsv) at the Hanging Mercury Drop Electrode (HMDE)
|Marco Guida, Maria Michela Salvatore and Francesco Salvatore*|
|Dipartimento di Biologia, Università degli Studi di Napoli “Federico II”, Via Cintia, 21-80126 Napoli, Italy|
|Corresponding Author :||Francesco Salvatore
Dipartimento di Biologia
Universitàdegli Studi di Napoli “Federico II”
Via Cintia, 21 - 80126 Napoli, Italy
Tel: 39 081 674389
E-mail: [email protected]
|Received August 20, 2015; Accepted September 04, 2015; Published September 11, 2015|
|Citation: Guida M, Salvatore MM, Salvatore F (2015) Riboflavin (Vitamin B2) Assay by Adsorptive Cathodic Stripping Voltammetry (Adcsv) at the Hanging Mercury Drop Electrode (HMDE). Biochem Physiol 4:177. doi: 10.4172/2168-9652.1000177|
|Copyright: © 2015 Guida M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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In this study the interactions of Riboflavin (Vitamin B2) with a mercury surface are investigated. Firstly, by using Cyclic Voltammetry, it is demonstrated that Riboflavin can be efficiently accumulated, by adsorption from buffered solutions containing an excess of NaClO4, onto the mercury drop of a HMDE. Secondly, it is shown that the adsorbed Riboflavin can be reduced through an electrochemical reaction whose stoichiometry is extricated by confronting simulated with experimental CV voltammograms acquired in a range of pH between about four and nine. Finally, the cathodic current, sustained by the surface reduction of Riboflavin, is exploited for assaying Riboflavin via Differential Pulse Adsorption Cathodic Stripping Voltammetry (DP AdCSV) within the frame of the standard additions calibration procedure.
By applying the suggested DP AdCSV procedure with standard voltammetric equipment, typical DP settings and pre-electrolysis time of about 10 s, a linear response is maintained if Riboflavin concentration in the electrolysed solution does not exceed about 2 mg/l. On the other side, a limit of detection (expressed as the concentration of Riboflavin in the electrolysed solution) of 7 μg/l has been achieved with a pre-electrolysis time of 68 s.