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RNA Preservation and Stabilization

Zahra Salehi1 and Mohammad Najafi2*
1Medical Biotechnology Department, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
2Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran
*Corresponding Author : Mohammad Najafi
Department of Biochemistry, Iran University of Medical Sciences
Tehran, Iran
Tel: +985118673107
E-mail: nbsmmsbn@iums.ac.ir/td>
Received January 02, 2014; Accepted February 04, 2014; Published February 10, 2014
Citation: Salehi Z, Najafi M (2014) RNA Preservation and Stabilization. Biochem Physiol 3:126. doi:10.4172/2168-9652.1000126
Copyright: © 2014 Salehi Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

RNA quality and integrity is a prominent issue in gene expression analysis which emerged as a critical tool in life science researches, drug discovery and optimization of bioproduction. Handling and preservation methods including formalin fixation, flash freezing and chemical preservatives (sulfate solution and TRIzol) and commercial compounds (RNAlater, Allprotect and PAXgene) are widely applied to keep high quality RNA within fresh tissue samples. In this article, we tried to give a general idea on basic aspects of the above methods.

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