Agrobacterium Mediated Genetic Transformation In Solanum Surattense Burma.F. From Leaf Explants | 4793
Journal of Biotechnology & Biomaterials
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Genetic transformation studies have been carried out in Solanum surattence Burma.f through Agrobacterium tumefaciens
mediated transformation mature and healthy leaves were selected from the plants grown in green house for the present
experiment. The explants were cultured over night on a one-day-old feeder layers. These overnight cultured leaf disc explants
were co-cultivated with A.tumefaciens strain LBA 4404 harboring a binary vector containing the B-glucoronidase (GUS) and
neomycin phosphotransferase (Npt-11) genes. The leaf disc explants were transferred to selective regeneration medium. The
explants began to regenerates 3 weeks after transformation and Kanamycin resistant shoots have been developed. Shoot developed
after transformation showed the GUS assays. Amplified DNA fragment was produced in PCR using N-11 specific primer and
DNA from the transgenics. The transgenic plants were also confirmed using southern blotting technique. The efficiency of
protocol and role of Agrobacterium mediated transformation in crop improvement will be discussed.
T. Ugandhar has completed his Ph.D at the age of 25 years from Kakatiya University under the guideness of Prof N. Ramaswamy Head Department
of Biotechnology Kakatiya University Warangal immediately he was started his committed teaching career as a Lecturer in Botany at C.V. Raman
P.G. College Mancherial in 2005 and he was appointed as Assistant Professor by APPSC at S.R.R Govt Degree and P.G. College Karimnagar. He
has published 25 research articles, co-authored 2 books and guided 10 M.Phil theses. He has attended 15 national seminars and 3 international
seminars and also attends 4 symposia and workshops. His major fields of teaching and research include Cytology and Cytogenetics, Genetics
and Plant Breeding, Molecular Genetics, Mutation Breeding, Plant Tissue Culture and Biotechnology. He successfully applied mutation breeding to
brinjals, chillies and tomatoes and developed several agronomically useful varieties. He established clonal multiplication of certain forest trees. He
has a patent pending for In Vitro Propagation of Tassar Silk Plant.
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