Reach Us +44-3308187254


Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Can Interference Of GIP Activity Modulate GPCRs For Therapeutic Gains? | 13273
ISSN: 2167-065X

Clinical Pharmacology & Biopharmaceutics
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 642

Clinical Pharmacology & Biopharmaceutics received 642 citations as per Google Scholar report

Clinical Pharmacology & Biopharmaceutics peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Genamics JournalSeek
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • Publons
  • Euro Pub
Share This Page

Can interference of GIP activity modulate GPCRs for therapeutic gains?

International Summit on Clinical Pharmacy & Dispensing

Kenneth A. Chinkwo, Ian M. Coupar Erica Sloan and Helen R. Irving

ScientificTracks Abstracts: Clinic Pharmacol Biopharmaceut

DOI: 10.4172/2167-065X.S1.002

GPCRs are large family of cell surface proteins participating in signal transduction where C-terminus recruits GPCR Interacting Proteins (GIPs) that regulate GPCR function. The 5-HT 4 (a, d, e, f and g) receptor (R) splice variants possess canonical type 1 or type 2 PDZ domains predicted to interact with various GIPs which could influence their modulatory and prokinetic functions in the intestine. This study investigates the distribution of 5-HT 4 R and GIPs in the guinea pig intestine and to determine if human 5-HT 4 R and specific GIPs interact using in vitro cell system. RT-PCR, western and immunoflourescence analysis were used to investigate transcript and protein expression. N-terminal FLAG tagged 5-HT 4 R variants and Lin 7 homologues (h) with C-terminal tagged V5, c-Myc and HA constructs were generated and expressed in COS-7 cells to study interactions. The 5-HT 4 R, GRKs and Lin 7h were expressed in the guinea pig intestine. Lin 7h and 5-HT 4 R co-localized in cell lines and the 5-HT 4 aR and Lin 7h were co-immunoprecipitated. We have previously shown that 5-HT 4 R and GIPs, GRKs and Lin7h, are found in the human colon. Our data indicates that this occurs in the guinea pig and we demonstrate that it may be possible for 5-HT 4 R and Lin 7h to interact in the same cells. The results suggest Lin 7h could be a potential target to modulate 5-HT 4 R function. This would be of particular relevance when 5-HT 4 R variant expression is altered as demonstrated in cancerous tissue (Cartier et al. 2005).

Kenneth Chinkwo completed his Ph.D. degree in Molecular Pharmacology at Monash University Australia in 2011. He studied serotonin receptors and G-protein coupled receptor (GPCR) interacting proteins; their role as potential therapeutic targets in clinical medicine. Ken holds a B.Sc. hons in Medical Laboratory Sciences at the University of Calabar Teaching hospital (UCTH) Nigeria. He worked in several pathology laboratories in Cameroon his home country. Ken obtained a Masters Degree in Biochemistry in the area of cancer biology in the University of the Western Cape, South Africa. Currently, he is a Medical Science lecturer in Charles Sturt University (CSU) Australia.