Detection Of Cyclospora, Microsporidia And Cryptosporidium By Direct Microscopy And PCR In Stools Specimens In Northwest Iran | 4702
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

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Detection of Cyclospora, Microsporidia and Cryptosporidium by direct microscopy and PCR in stools specimens in northwest Iran

3rd World Congress on Biotechnology

Shahrokh Izadi, Abbas Shahbazi, D Leshan Wannigama, Shirzad Gholami, Nasrin Mirsamadi, Iraj Nokhahi and Ardavan Ghazanchaii5

Posters: Agrotechnol

DOI: 10.4172/2155-952X.S1.020

This study directs to Cyclospora, Microsporidia and Cryptosporidium three parasitic protozoa may be spread in food or water, even if are not considered as huge a risk to food production, owing to the fact that in cold and humid conditions, they may stay alive for months, control of them is important and following of consumption of contaminated drinking water or foods that have not received heat treatment, some outbreaks may appear. In this study we assessed the frequency of the two parasites in fecal samples submitted to the Central Parasitology laboratory of Tabriz, Iran. From 2009 to 2010, 1825 fecal samples submitted to the Central Parasitology laboratory of Tabriz were assessed by conventional methods of Light microscopy, Formalin-ether concentration technique, staining technique and PCR method. The frequency of Cryptosporidium was 0. 0%, 15%, 3% and 2% and Microsporidia were 0.0%, 3%, 1% and 0% by methods of microscopy, formalin-ether concentration, staining and PCR, respectively. Cyclospora was not found in all the stool samples examined. The frequencies of these three protozoa in our study were less than our expectations. Lack of infection with Cyclospora can be good news for local health care providers. More investigations especially in at risk populations is needed for health policy making.
Shahrokh Izadi is a Lecturer in Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran. He was graduated in Public Health Health in BSc and also postgraduate in Medical Parasitology. He has 12 published articles in different international journals and also 8 published in different congress around the world.