Clinical Pharmacology & Biopharmaceutics
Open Access

Abstract

Background & Aim: The aim of this study is to evaluate the effects of tree Mediterranean molluscs co-product protein extracts on human glioblastoma U87 and HeLa cell line epithelia cervix carcinoma. Hypobranchial gland proteins (HGPE) are extracted from the gastropods Hexaplex trunculus (HT) and Bolinus brandaris (BB). The squid ink proteins are extracted from the cephalopod Sepia officinalis. Methods: Proteins are extracted by acetone precipitation. Cell viability is measured using MTT assay. Cell adhesion and migration are established using fibrinogen as matrix. Results: Both HGPE HT and BB are non-cytotoxic substances until 20 mg/ML. They decrease by more than 50% at 25 mg/mL. All HGPE significantly impair migration of U87 cells towards fibrinogen in a concentration dependent manner. Concentrations for 50% inhibition (IC50) of male and female HGPE HT are of 3.7 and 4 mg/mL, respectively. They are of 4.2 and 5.8 mg/ml for male and female HGPE BB, respectively. Squid ink proteins block the migration of U87 to fibrinogen in a dose dependent manner. The IC50 is about 9.2 �?¼g/mL. This supernatant also inhibits cell adhesion U87 on various types of matrices. Inhibitions are 60% fibrinogen and 25% fibronectin. Similarly, HGPE of both HT and BB inhibits HeLa cell adhesion to fibrinogen at 50 mg/mL. Male and female inhibitions significantly impair at 10 mg/mL and continue until 20 mg/mL. Squid ink proteins inhibit also HeLa cell adhesion. Inhibition significantly impairs at 10 mg/ML and continues until 30mg/mL. Conclusion: HGPE HT, HGPE BB and squid ink proteins may have the potential to serve as a model for future anticancerdrugs development.

Biography

Email: soufiaellouzchabchoub@yahoo.fr