alexa Effects Of Hypobranchial Glands And Squid Ink Protein Extracts From Three Mediterranean Molluscs On Human Glioblastoma U87 And HeLa Cell Line Epithelia Cervix Carcinoma
ISSN: 2167-065X

Clinical Pharmacology & Biopharmaceutics
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

4th International Pharma & Clinical Pharmacy Congress
November 07-09, 2016 Las Vegas, Nevada, USA

Chabchoub Ellouze Soufia, Ben Mabrouk Hazem, Sayari Nejia and Marrakchi Naziha
Higher Institute of Medical Technologies of Tunis, Tunisia
Posters & Accepted Abstracts: Clin Pharmacol Biopharm
DOI: 10.4172/2167-065X.C1.023
Abstract
Background & Aim: The aim of this study is to evaluate the effects of tree Mediterranean molluscs co-product protein extracts on human glioblastoma U87 and HeLa cell line epithelia cervix carcinoma. Hypobranchial gland proteins (HGPE) are extracted from the gastropods Hexaplex trunculus (HT) and Bolinus brandaris (BB). The squid ink proteins are extracted from the cephalopod Sepia officinalis. Methods: Proteins are extracted by acetone precipitation. Cell viability is measured using MTT assay. Cell adhesion and migration are established using fibrinogen as matrix. Results: Both HGPE HT and BB are non-cytotoxic substances until 20 mg/ML. They decrease by more than 50% at 25 mg/mL. All HGPE significantly impair migration of U87 cells towards fibrinogen in a concentration dependent manner. Concentrations for 50% inhibition (IC50) of male and female HGPE HT are of 3.7 and 4 mg/mL, respectively. They are of 4.2 and 5.8 mg/ml for male and female HGPE BB, respectively. Squid ink proteins block the migration of U87 to fibrinogen in a dose dependent manner. The IC50 is about 9.2 μg/mL. This supernatant also inhibits cell adhesion U87 on various types of matrices. Inhibitions are 60% fibrinogen and 25% fibronectin. Similarly, HGPE of both HT and BB inhibits HeLa cell adhesion to fibrinogen at 50 mg/mL. Male and female inhibitions significantly impair at 10 mg/mL and continue until 20 mg/mL. Squid ink proteins inhibit also HeLa cell adhesion. Inhibition significantly impairs at 10 mg/ML and continues until 30mg/mL. Conclusion: HGPE HT, HGPE BB and squid ink proteins may have the potential to serve as a model for future anticancerdrugs development.
Biography

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords