Reach Us +44-3308187254


Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Evaluation Of Intestinal Permeability And Efflux Of Lamivudine Using Ex Vivo Method In Franz Cells | 61751
ISSN: 2167-065X

Clinical Pharmacology & Biopharmaceutics
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 642

Clinical Pharmacology & Biopharmaceutics received 642 citations as per Google Scholar report

Clinical Pharmacology & Biopharmaceutics peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Genamics JournalSeek
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • Publons
  • Euro Pub
Share This Page

Evaluation of intestinal permeability and efflux of lamivudine using ex vivo method in Franz cells

4th International Pharma & Clinical Pharmacy Congress

Andre Bersani Dezani, Thaisa Marinho Dezani and Cristina Helena dos Reis Serra

University of Sao Paulo, Brazil

ScientificTracks Abstracts: Clin Pharmacol Biopharm

DOI: 10.4172/2167-065X.C1.022

For orally administered drugs, the bioavailability is an important parameter related to the absorption process. In the gut, most of compounds are transported mainly by trans-cellular passive diffusion. However, the presence of efflux transporters, as P-glycoprotein (P-gp), can interfere on the absorption process. Lamivudine (3TC) is widely used for HIV treatment and its bioavailability has been reported as variable (around 80%). Based on that, the intestinal permeability and efflux were evaluated for 3TC using ex vivo method in Franz cells. Male Wistar rats were anesthetized and a portion of jejunum was mounted in the Franz cells. The experiments were performed considering the direction apical (A) to basolateral (B). A Ringer-Krebs- Hepes modified solution was used as transport media and 3TC was solubilized for permeability studies while 3TC with P-gp inhibitor verapamil were solubilized for efflux experiments. The transport media with drug or drug with verapamil was added in the donor compartment and samples were collected from the receptor chamber. The viability of all intestinal membranes was checked by trans-epithelial electrical resistance (TEER) before and after experiments. Metoprolol was used as marker of high permeability. The apparent permeability (Papp) of 3TC was 1.26 (± 0.27)x10-5 cm/s and for 3TC with verapamil was 3.77 (±1.1)x10-5 cm/s. These results suggest that 3TC is a P-gp substrate. In the literature, 3TC is a weak P-gp substrate in MDCKMDR1, which corroborates with the results obtained in Franz cells. The differences between results of 3TC and 3TC with verapamil suggest that the antiretroviral is a P-gp substrate, which may be related to its variable bioavailability. In addition, the study showed that the Franz cells device can be used for efflux studies.

Andre Bersani Dezani has completed his Master’s degree in 2010 and since then, he is completing his PhD thesis related to permeability studies using different methods as ex vivo, in situ and in vitro models. His research field also includes “Solubility, biopharmaceutical classification systems (BCS and BDDCS), dissolution studies and ADME prediction”. He was a Visiting Scholar at Benet Lab, University of California San Francisco, USA.

Email: [email protected]