alexa Evaluation Of Intestinal Permeability And Efflux Of Lamivudine Using Ex Vivo Method In Franz Cells
ISSN: 2167-065X

Clinical Pharmacology & Biopharmaceutics
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

4th International Pharma & Clinical Pharmacy Congress
November 07-09, 2016 Las Vegas, Nevada, USA

Andre Bersani Dezani, Thaisa Marinho Dezani and Cristina Helena dos Reis Serra
University of Sao Paulo, Brazil
ScientificTracks Abstracts: Clin Pharmacol Biopharm
DOI: 10.4172/2167-065X.C1.022
Abstract
For orally administered drugs, the bioavailability is an important parameter related to the absorption process. In the gut, most of compounds are transported mainly by trans-cellular passive diffusion. However, the presence of efflux transporters, as P-glycoprotein (P-gp), can interfere on the absorption process. Lamivudine (3TC) is widely used for HIV treatment and its bioavailability has been reported as variable (around 80%). Based on that, the intestinal permeability and efflux were evaluated for 3TC using ex vivo method in Franz cells. Male Wistar rats were anesthetized and a portion of jejunum was mounted in the Franz cells. The experiments were performed considering the direction apical (A) to basolateral (B). A Ringer-Krebs- Hepes modified solution was used as transport media and 3TC was solubilized for permeability studies while 3TC with P-gp inhibitor verapamil were solubilized for efflux experiments. The transport media with drug or drug with verapamil was added in the donor compartment and samples were collected from the receptor chamber. The viability of all intestinal membranes was checked by trans-epithelial electrical resistance (TEER) before and after experiments. Metoprolol was used as marker of high permeability. The apparent permeability (Papp) of 3TC was 1.26 (± 0.27)x10-5 cm/s and for 3TC with verapamil was 3.77 (±1.1)x10-5 cm/s. These results suggest that 3TC is a P-gp substrate. In the literature, 3TC is a weak P-gp substrate in MDCKMDR1, which corroborates with the results obtained in Franz cells. The differences between results of 3TC and 3TC with verapamil suggest that the antiretroviral is a P-gp substrate, which may be related to its variable bioavailability. In addition, the study showed that the Franz cells device can be used for efflux studies.
Biography

Andre Bersani Dezani has completed his Master’s degree in 2010 and since then, he is completing his PhD thesis related to permeability studies using different methods as ex vivo, in situ and in vitro models. His research field also includes “Solubility, biopharmaceutical classification systems (BCS and BDDCS), dissolution studies and ADME prediction”. He was a Visiting Scholar at Benet Lab, University of California San Francisco, USA.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords