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High Performance Liquid Chromatographic Method For Determination Of Garenoxacin Mesylate In Pharmaceutical Dosage Form | 4623
Journal of Biotechnology & Biomaterials
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A simple and rapid HPLC method was developed for the determination of Garenoxacin mesylate, in pharmaceutical dosage form. An Agilent 1100 series HPLC with DAD Detection and column thermostat was used for the proposed method.
Chromatographic separation was achieved on a YMC Pack Pro; C8(150 x 4.5 mm, 5μm) column with mobile phase containing buffer (1.36g KH2
PO4 in 1000ml water, 1ml TEA, pH 4.5?0.05 with OPA): acetonitrile (75:25%, v/v) at a flow rate of 1.0 ml/min. The run time was 6 minutes. The column temperature was maintained at 10?
C.Diode array detection and quantification of Garenoxacin was carried out at 279nm.The drug shows linearity in the concentration range of 10-160 μg/ml with a correlation co-efficient of
0.9993.The developed method was validated for specificity, linearity, accuracy, precision (system and method),solution stability and
system suitability. Specificity of the method was evaluated with respect to identification, placebo interference and known impurity
interference. The proposed method was used successfully for determination of Garenoxacin mesylate in tablet dosage form and can
be further applied for bioavailability and pharmacokinetics studies.
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