Isolation And Identification Of Lactobacillus Sp From Milk And Yoghurt, PCR Amplification For The Amplification Of Lactase Encoding Gene | 4670
Journal of Biotechnology & Biomaterials
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Lactase is an intracellular enzyme in Lactobacillus sp which has tremendous potential in research and applications on various
fields like food, bioremediation, etc. This study was carried out to detect the lactase encoding gene in Lactobacillus sp by
polymerase chain reaction, for this three Lactobacillus sp (LB1, LB2 and LB3) were isolated and identified from raw milk and
yoghurt. The identities of the isolate were based on the morphological, biochemical characteristics of Lactobacillus as given in
Bergy?s manual of determinative bacteriology. Carbohydrate utilization of the bacteria was studied by UV/VIS spectrophotometer
with an initial concentration of 4.8mg/ml and after 28hrs the sugar was reduced to 3.8mg/ml. Antibiotic sensitivity test was
conducted and observed that all the three isolates were resistant to Ampicillin(A
10), Pencillin(P2), Methicillin(M5) and sensitive
resistant to Ciproflaxin(CF5), Tetracyclin(T30)and Streptomycin(S
10). Lactobacillus casei was used as the control sample for all the tests. The Lactase encoding gene was amplified by Polymerase Chain Reaction and bands were observed at 0.5Kbp on Agarose gel
electrophoresis using 500bp as the marker DNA indicating that the gene was encoded in the chromosome.
Niti Sarat completed her Post graduation in M.tech Biotechnology from SRM University in the year 2010. She is currently working as Analyst in
Biotechnology in Quality Assurance Laboratories, Quilon, Kerala. She is leading the team of Biotechnologist in the department. She has supervised
the dissertation thesis of more than 20 graduates and post graduates in the field of Life Science till now. She has sequence publication in NCBI
GENBANK databases to her credit.
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