alexa Label-free Proteomic Analysis Of Environmental Acidification-influenced Streptococcus Pyogenes Secretome Reveals A Novel Acid-induced Protein Histidine Triad Protein A (HtpA) Involved In Necrotizing Fasciitis | 23501
ISSN: 2167-065X

Clinical Pharmacology & Biopharmaceutics
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

2nd International Summit on Clinical Pharmacy

Pao-Chi Liao
ScientificTracks Abstracts: Clinic Pharmacol Biopharm
DOI: 10.4172/2167-065X.S1.006
Abstract
Streptococcus pyogenes is a human pathogen responsible for various diseases. To cause diseases, S. pyogenes must adapt in adverse environments, such as acidic environment in the wound. The acid stimuli may trigger S. pyogenes invading from a mucosal to subepithelial tissue. However, how acid stimulates S. pyogenes to manipulate secretome for causing invasive infection is unclear. To investigate secretome change under acidic environment, a comparative secretomics by label-free LC-MS/MS was used to analyze the secretome from acidic and neutral conditions. The growth curves of S. pyogenes in acidic and neutral conditions are similar, which reveals that S. pyogenes can grow well during environmental acidification. The protein patterns on SDS-PAGE show prominent dissimilarity between the secretomes in acidic and neutral conditions. It demonstrates protein secretion is influenced by acid stress. The dynamic label-free LC-MS/MS profiling identified 172 proteins which are influenced by environmental acidification. Among these, 45 (28%) the identified proteins are predicted secreted proteins. Interestingly, the predicted secreted proteins occupy about 90% of protein abundance of secretome in acidic condition at stationary phase. In contrast, only 30% shows in neutral condition. It exhibits that acid is crucial for secreted protein expression. There are 21 pathogenesis-related secreted proteins effecting immune evasion, hemolysis, adhesion, tissue damage, and nutrient acquisition. The 24 non-pathogenesis-related secreted proteins could be potential virulence factors involved in invasive infection. Two known acid-induced proteins, SpeB and Pilin, are also observed. Several novel candidates, such as streptococcal histidine triad protein, CAMP, and biofilm regulatory protein A, are of special interests. This investigation provided key information for elucidating the broad influences and underlying mechanisms related to acidified environment for group A streptococcal infection.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

kactakapaniyor.com

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

Taktube

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

porn sex

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

Gaziantep Escort

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

sikiş

[email protected]

1-702-714-7001Extn: 9037

instafollowers

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

mp3 indir

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

putlockers

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

seks

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7