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Background: Proliferative Diabetic Retinopathy (PDR) is one of the leading causes of blindness. The role of microRNA-
200b (miRNA-200b) in the pathogenesis of PDR has been suggested in diabetic animal models. The aim of this study was to
assess miRNA-200b expression level for the first time in the vitreous of patients with PDR and to study its relation to vascular
endothelial growth factor (VEGF) as one of the pathogenic mechanisms in PDR.
Methods: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure miRNA-200b
expression in the vitreous samples obtained from 29 eyes with PDR and from 30 eyes with idiopathic macular hole, as a control
group. In addition, enzyme linked immunosorbent assay was used to measure VEGF in these vitreous samples.
Results: MicroRNA-200b expression was increased by about 5-folds in the vitreous samples from eyes with PDR compared with
the controls (P=<0.001). Logistic regression analysis revealed for the first time that vitreous miRNA-200b was an independent
risk factor for the development of PDR. VEGF level in the vitreous was significantly higher than controls (P=<0.001), but no
significant correlation was found between miRNA-200b and VEGF.
In Conclusion: MiRNA-200b and VEGF were significantly increased in the vitreous of eyes with PDR, but in a non-correlated
pattern. Overexpressed miRNA-200b independently increased the risk of PDR occurrence. Further studies are needed to
identify the miRNA-200b targeted genes involved in the pathogenesis of PDR and examine the potential role of miRNA-200b
as a target for PDR treatment.
Faculty of Ophthalmology at Alexandria University, Egypt.