alexa Permeability Study Using The Single-pass Intestinal Perfusion In Rats: Is The Zidovudine A Substrate For P-gp?
ISSN: 2167-065X

Clinical Pharmacology & Biopharmaceutics
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

4th International Pharma & Clinical Pharmacy Congress
November 07-09, 2016 Las Vegas, Nevada, USA

Thaisa Marinho Dezani, Andre Bersani Dezani and Cristina Helena dos Reis Serra
University of Sao Paulo, Brazil
ScientificTracks Abstracts: Clin Pharmacol Biopharm
DOI: 10.4172/2167-065X.C1.022
Abstract
The small intestine is the main site of absorption for many drugs orally administered and it depends on solubility and permeability characteristics of the compound to reach the bloodstream. Many drugs are absorbed mainly by trans-cellular passive diffusion, but the presence of efflux transporters, such as P-glycoprotein (P-gp) can hamper the permeation. The oral bioavailability of zidovudine (AZT) is not complete and among other factors, efflux transport can be involved on its permeation process. Thus, this study aims to evaluate the permeability of AZT and its interaction with P-gp using the single-pass intestinal perfusion (SPIP) in rats. Male Wistar rats were anesthetized with ketamine-xylazine mixture. Blank perfusion solution pH 6.5 at 37°C was pumped into jejunum to clean any residual debris. Then, the perfusion solution containing AZT was pumped into intestine and samples were collected from the distal portion. The same procedure was made for AZT with P-gp inhibitor verapamil and all samples were quantified by HPLC method. Metoprolol and ranitidine were used as permeability marker substances in this study. The effective permeability (Peff) of AZT was 4.37 (±0.45)x10-5 cm/s. For AZT with verapamil, the Peff was 5.44 (±0.39)x10-5 cm/s. These results showed that AZT has an interaction with P-gp. That is in accordance with other in vitro and ex vivo studies reported in the literature, which can explain the variability on the oral bioavailability. The comparison between AZT and AZT with verapamil results led to conclude that AZT had its permeability increased when verapamil was used. Thus, AZT can have an interaction with P-gp which may influence on its permeability and contribute for its incomplete bioavailability.
Biography

Thaisa Marinho Dezani has completed her Master’s degree in 2012 and since then, she is completing her PhD thesis related to permeability studies using different methods as in situ, ex vivo and in vitro models. Her research field also includes “Solubility, biopharmaceutical classification systems (BCS and BDDCS), dissolution studies and ADME prediction”. She was a Visiting Scholar at Benet Lab, University of California San Francisco, USA.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version